The sequence and molecular weight of the MISSION siRNA Universal Negative Controls are considered proprietary. These products are lyophilized from solution. The mass per vial and subsequent ng/mL concentration following reconstitution are not available.
SIC001
MISSION® siRNA Universal Negative Control #1
Synonym(s):
MISSION® siRNA Universal Negative Control
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About This Item
NACRES:
NA.51
UNSPSC Code:
41105324
Pricing and availability is not currently available.
product line
MISSION®
storage temp.
−20°C
Application
MISSION® siRNA Universal Negative Control #1 has been used as a negative control for siRNA transfections.[1]
Negative controls are an essential part of every siRNA experiment, and here we offer two sizes for your convenience. This negative control has been tested in vitro in Human, Mouse, and Rat cells.
Legal Information
All siRNA oligos are sold under license from the Massachusetts Institute of Technology.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| product line MISSION® | product line MISSION® | product line MISSION® | product line MISSION® |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the ng/mL of a 100 uM solution of 10nmol Mission siRNA universal negative control #1?
1 answer-
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Buongiorno quale siRNA negative control (#1 o #2) consigliate di usare? o forse meglio usarli insieme (#1 e #2)? Avete dei protocolli da seguire? Grazie mille
1 answer-
SIC001 and SIC002 are both unconjugated negative controls. They have different sequences. Either one can be used - we just provided more than one option. A typical experiment uses 1 negative control. Please see the citation below as an example:
Hudson W Coates, Isabelle M Capell-Hattam, Ellen M Olzomer, Ximing Du, Rhonda Farrell, Hongyuan Yang, Frances L Byrne, Andrew J Brown (2023) Hypoxia truncates and constitutively activates the key cholesterol synthesis enzyme squalene monooxygenase eLife 12:e82843. https://doi.org/10.7554/eLife.82843
Please see our full selection of siRNA Negative Controls at this link: https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/next-gen-sequencing/mission-sirna-universal-negative-controls
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I just purchase one 10nmol Mission siRNA universal negative control #1, but I got the product is dried, How to handle / resuspend it ?
1 answer-
Negative siRNA controls are shipped dry as duplexes and should be resuspended in DNAase, RNAase-free water.
The 10 nmol quantity should be resuspended with 100 microliters of water, while the 1 nmol tubes should be resuspended with 10 microliters.
These volumes will result in the buffer being at 1x concentration and the siRNA at a concentration of 100 micromolar (100 pmol per microliter).
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