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SAB4600239

Sigma-Aldrich

Anti-Mouse IgG2a (γ2a), CF488A antibody produced in goat

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

CF 488A conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

concentration

~2 mg/mL

technique(s)

flow cytometry: 1-10 μg/mL
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
indirect immunofluorescence: 1-10 μg/mL
western blot: suitable

fluorescence

λex 490 nm; λem 515 nm

storage temp.

−20°C

target post-translational modification

unmodified

General description

CF dye goat anti-mouse IgG isotype-specific secondary antibody conjugates are labeled with our exceptionally bright, photostable CF dyes. The conjugates are prepared from affinity-purified antibodies that react with Fc portion of the heavy chain of mouse IgG1, IgG2a, or IgG2b. To minimize cross reactivity, the antibodies are cross-adsorbed against other mouse IgG subclasses (IgG2a, IgG2b, IgG3), and human, bovine and rabbit serum proteins.
IgG2a (immunoglobulin G2a) belongs to the class of IgG. In mouse, IgG2a is the major serum antibody.

Specificity

Binds with the Fc portion of mouse IgG2a but not with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.

Immunogen

mouse IgG2a (H+L)

Biochem/physiol Actions

IgG2a (immunoglobulin G2a) is considered as an efficient antibody produced against viral and bacterial infections. IgG2a expression is induced by viral infection. IgG2a offers host defense through activation of complement system. This antibody has specificity towards Fc receptors expressed on phagocytes and stimulates antibody-dependent cell mediated cytotoxicity. IgG2a also induces macrophages mediated opsonophagocytosis, which has been observed in response to influenza virus and leads to its clearance from the infected cells.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 0.2% BSA.

Preparation Note

Protect from light. The antibody solution should be gently mixed before use.

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
CF is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Fc?RIV deletion reveals its central role for IgG2a and IgG2b activity in vivo
Nimmerjahn F, et al.
Proceedings of the National Academy of Sciences of the USA, 107(45), 19396-19401 (2010)
Francesco Mascadri et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 69(8), 535-541 (2021-07-21)
Immunodetection on mouse routinely processed tissue via antibodies raised in mice faces cross-reactivity of the secondary anti-mouse reagents with endogenous immunoglobulins, which permeate the tissue. Various solutions to this problem have been devised and include endogenous Ig block with anti-mouse
Fernando Rodriguez-Caro et al.
Molecular biology and evolution, 38(11), 5021-5033 (2021-07-30)
Sexually dimorphic development is responsible for some of the most remarkable phenotypic variation found in nature. Alternative splicing of the transcription factor gene doublesex (dsx) is a highly conserved developmental switch controlling the expression of sex-specific pathways. Here, we leverage
Distinct contributions of vaccine-induced immunoglobulin G1 (IgG1) and IgG2a antibodies to protective immunity against influenza.
Huber V C, et al.
Clinical and Vaccine Immunology : CVI, 13(9), 981-990 (2006)
TLR9 signaling in B cells determines class switch recombination to IgG2a.
Jegerlehner A, et al.
Journal of Immunology, 178(4), 2415-2420 (2007)

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