SAB4200310

Anti-Granulin (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-Acrogranin, Anti-Epithelin, Anti-GEP, Anti-GP88, Anti-GRN, Anti-PCDGF, Anti-PEPI, Anti-PGRN, Anti-Progranulin
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~80 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 10-20 μg/mL using A431 cells; western blot: 1-2 μg/mL using MCF7 cell extracts
• UniProt accession no.: P28799
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... GRN(2896)

Description

General description

Granulin is composed of 7.5 repeat units of a unique, structurally defined cysteine-rich granulin-eptihelin motif.

Granulins (also known as granulin/epithelin precursor [GEP]) are a secreted growth factors expressed mainly by granulocytes. They are secreted as high molecular weight precursor proteins, pro-granulins (PGRN), with seven and a half repeats of 12-cysteine granulin motif. Five human granulins have been isolated and they have a common precursor. PGRN is highly expressed in rapidly proliferating epithelial cells such as keratinocytes. It is also synthesised in neurons of cerebral cortex, hippocampus and cerebellum, central and peripheral nervous system.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminus of human granulin precursor, conjugated to KLH.

Application

Anti-Granulin (C-terminal) antibody produced in rabbit has been used in immunoblotting, immunofluorescence and western blotting.

Biochem/physiol Actions

Granulins are potent mitogenic factors, and mediate proliferation, motility, wound healing and anchorage-dependent growth of fibroblasts. Some of the pathways that are associated with PGRN are MAPK and PI3K/Akt.

Progranulin gene is mitotically quiescent epithelia and is expressed in low levels. It stimulates the proliferation in epithelial cells and promotes their anchorage-independent growth. Overexpression of progranulin confers epithelial invasiveness and tumorigenicity. There is growing evidence that granulin is involved in embryonic and neonatal development, placenta, the epidermis, vasculature angiogenesis, and the developing nervous system. Granulin stimulates an increase in cyclins D1 and cyclins B. Granulin overexpression has been associated with many cancers, including glioblastoma, breast, renal and invasive ovarian cancers. Granulin is highly expressed in motor neurons, it regulates neurite outgrowth and promotes neuronal cell survival. Progranulin gene mutations and PGRN gene haploinsufficiency have been recently proposed to cause a form of frontotemporal dementia (FTLD). It is also associated with the formation of pathogenic Tar-DNA Binding Protein 43 (TDP-43) and ubiquitin positive inclusions.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable