SAB4200194

Anti-Pontin antibody, Mouse monoclonal

clone 5G3-11, purified from hybridoma cell culture

• Synonym(s): Anti-49 kDa TATA box-binding protein-interacting protein, Anti-49 kDa TBP-interacting protein, Anti-54 kDa erythrocyte cytosolic protein, Anti-ECP-54, Anti-INO80 complex subunit H, Anti-NMP 238, Anti-Pontin52, Anti-RUVBL1, Anti-RVB1, Anti-RuvB (e coli homolog)-like 1, Anti-RuvB-like 1, Anti-TAP54-alpha, Anti-TIH1, Anti-TIP49, Anti-TIP49A, Anti-TIP60-associated protein 54-alpha, Anti-nuclear matrix protein 238
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 5G3-11, monoclonal
• form: buffered aqueous solution
• mol wt: ~50 kDa
• species reactivity: canine, human, monkey
• technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; indirect immunofluorescence: suitable; western blot: 1:1,000-1:2,000 using whole extracts of human HEK-293T or MCF-7 cells
• isotype: IgG
• UniProt accession no.: Q9Y265
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RUVBL1(8607)

Description

General description

Monoclonal Anti-Pontin (mouse IgG1 isotype) is derived from the hybridoma 5G3-11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a human pontin fusion protein. Pontin and reptin are two related members of the AAA+ (ATPases associated with diverse cellular activities) family of ATPases. Pontin and reptin share some homology to the bacterial RuvB gene.

Immunogen

human Pontin fusion protein

Application

Monoclonal Anti-Pontin antibody produced in mouse has been used in:

• western blotting
• immunoprecipitation
• ChIP-sequencing
• immunohistochemistry
• immunofluorescence
• RNA immunoprecipitation

Biochem/physiol Actions

Pontin and Reptin are associated with several chromatin-remodeling complexes and are involved in multiple biological processes including chromatin remodeling, DNA damage repair, transcriptional regulation, telomerase activity, apoptosis and cancer metastasis. Both proteins are also involved in cellular transformation by beta-catenin and c-myc through their chromatin remodeling function.

Physical form

Solution in 0.01 M phosphate buffered saline, 7.4 pH, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable