RECOMT

Thrombin CleanCleave^™ Kit

• Synonym(s): Thrombin Cleavage Kit
• NACRES: NA.56
• UNSPSC Code: 12352200

Properties

• form: suspension
• Quality Segment: 200
• mol wt: 37 kDa
• technique(s): protein purification: suitable
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins

Application

For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.

Features and Benefits

• Fast, efficient cleavage in as little as 2 hours
• Thrombin is covalently bound to agarose for easy removal.
• The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
• Cleave tags even in the presence of 0.1% Triton^™, 1 M urea or 5 mM EDTA
• Thrombin-agarose is reusable.

Preparation Note

200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.

Legal Information

CleanCleave is a trademark of Sigma-Aldrich Co. LLC

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Kit Components Only

• Thrombin Cleavage Buffer, 10× 10 mL

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable