The capture and detection antibodies are mouse monoclonal. The immunogens are recombinant human Osteocalcin with glutathione s-transferase (GST). These detect the large NH2- terminal fragment and intact hOC. The specificity of activated vs. inactivated forms has not been determined.
RAB1073
Human Osteocalcin ELISA Kit
for cell culture supernatants, plasma, and serum samples
Synonym(s):
Osteocalcin
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About This Item
UNSPSC Code:
41116158
NACRES:
NA.32
Pricing and availability is not currently available.
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species reactivity
human
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable
input
sample type cell culture supernatant(s)
sample type serum sample(s)
sample type plasma
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.4 ng/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... BGLAP(632)
General description
Osteocalcin (OC/OCN) is a bone-building protein.[1] This γ-carboxylated protein is made up of 49 amino acids and is synthesized by osteoblasts, odontoblasts and hypertrophic chondrocytes.[2] The bone γ-carboxyglutamate protein (BGLAP), that produces OCN is located on human chromosome 1q22.[3]
This ELISA antibody pair detects human Osteocalcin. Other species are not determined.
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached Protocolfor details.
Please refer to the attached Protocolfor details.
Biochem/physiol Actions
Osteocalcin (OC/ OCN) plays a major role in the bone remodeling cycle, affecting bone mineralization. It is considered as a marker of bone formation. OCN guards against the progression of diabetes and the metabolic syndrome.[2]
Other Notes
A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Met. Corr. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Ammar Nebigh et al.
Pediatric exercise science, 29(4), 513-519 (2017-09-06)
The aim of this study was to examine the relationship between bone mass and bone turnover markers with lean mass (LM) in pubescent soccer players. Two groups participated in this study, which included 65 elite young soccer players who trained
Bernhard Bielesz et al.
Scientific reports, 7(1), 17941-17941 (2017-12-22)
Vascular calcification is a component of cardiovascular disease, which is leading cause of death in patients with chronic kidney disease (CKD). A functional assay (T50-test) measuring the propensity of human serum to calcify associates with mortality and cardiovascular events in
Decreased Levels of Circulating Carboxylated Osteocalcin in Children with Low Energy Fractures: A Pilot Study
Popko J, et al.
Nutrients, 10(6), 734-734 (2018)
Jie Zhu et al.
Journal of diabetes and its complications, 31(8), 1354-1359 (2017-06-11)
This study presents nine patients with mitochondrial tRNA Leu (UUR) m.3243A>G mutation and compares the clinical characteristics and diabetes complications with type 1 diabetes (T1DM) or early onset type 2 diabetes (T2DM). The study covers 9 patients with MIDD, 33
Kevin D Cashman et al.
Nutrients, 11(8) (2019-07-31)
Reformulation of bread in terms of salt content remains an important measure to help achieve a reduction in salt intake in the population and for the prevention of hypertension and elevated blood pressure (BP). Our fundamental studies on the reduction
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Good day, I would like to know what molecule this kit detects, carboxylated osteocalcin or subcarboxylated osteocalcin?
1 answer-
Helpful?
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Does the RAB1073 Kit detect both activated and inactivated osteocalcin, or just one of them?
1 answer-
The Capture and Detection Antibodies are of the mouse monoclonal type. The immunogens are derived from recombinant human Osteocalcin with glutathione s-transferase. These antibodies are capable of detecting the large NH2-terminal fragment and intact hOC. However, the specificity of activated versus inactivated forms has not been tested.
Helpful?
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Does this Human Osteocalcin Kit measure total osteocalcin level, meaning the sum of undercarboxylated and carboxylated Osteocalcin? or a specific type?
1 answer-
We have not tested this ELISA kit for specificity of undercarboxylated and carboxylated Osteocalcin; here is information about capture and detection antibodies used in the kit:
Capture and Detection Antibodies are mouse monoclonal.
Immunogens: Recombinant human Osteocalcin with glutathione s-transferase.
Detects the large NH2- terminal fragment and intact human osteocalcin.
The specificity of activated vs. inactivated forms is not tested.Helpful?
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I am writing this message to ask for a detail protocol of RAB1073 (Human Osteocalcin ELISA). I have checked your website and I could not find a detail protocol to process my experiment. Could you please send me one PDF?
1 answer-
The protocol is attached to the lot-specific Certificate of Analysis.
Please see the sample Certificate below:
https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/SigmaABNO/RAB1073_1221J2004.pdfHelpful?
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