P7777

Poly(ethylene glycol)

Hybri-Max^™, mol wt 1,300-1,600, waxy solid, BioReagent, suitable for hybridoma

• Synonym(s): PEG
• Linear Formula: H(OCH₂CH₂)_nOH
• CAS Number: 25322-68-3
• NACRES: NA.75
• PubChem Substance ID: 24898877
• UNSPSC Code: 12352104
• MDL number: MFCD00081839
• Technique(s): cell culture | hybridoma: suitable

Properties

• biological source: synthetic (organic)
• Quality Segment: 200
• grade: Hybri-Max^™
• sterility: autoclaved
• product line: BioReagent
• form: waxy solid
• mol wt: 1,300-1,600
• technique(s): cell culture | hybridoma: suitable
• impurities: endotoxin, tested
• viscosity: 25-35 cP (210 °F)
• mp: 40-50 °C
• solubility: water: soluble (5 mL water/vial at 45 °C)
• SMILES string: C(CO)O
• InChI: 1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
• InChI key: LYCAIKOWRPUZTN-UHFFFAOYSA-N

Description

Application

Recommended for cell fusion.

Preparation Note

Melt the PEG at 45 °C or higher in a waterbath. Dilute to 30-50% using sterile, pre-warmed serum-free medium (e.g., for a 50% solution add 5 mL to the 5 g vial of PEG). The pH of the solution should be slightly alkaline (pink, not orange or purple). Adjustment of the pH with sterile NaOH may be required. Store solution below 0 °C. Prolonged storage or repeated freezing and thawing is not recommended.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): 281.5 °F - closed cup
• Flash Point (°C): 138.6 °C - closed cup
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)