This product is not tested for solubility. However, similar alkaline phosphatase products are prepared in 10 mM Tris-HCl, 50 mM NaCl, 1.5 mM MgCl2, 0.2% Zwittergent 3-14, pH 7.4.
P7640
Phosphatase, Alkaline from bovine intestinal mucosa
lyophilized powder, ≥10 DEA units/mg solid
Synonym(s):
Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
42010105
EC Number:
232-631-4
NACRES:
NA.54
MDL number:
Specific activity:
≥10 DEA units/mg solid
Biological source:
bovine intestinal mucosa
Pricing and availability is not currently available.
biological source
bovine intestinal mucosa
Quality Segment
form
lyophilized powder
specific activity
≥10 DEA units/mg solid
mol wt
dimer ~160 kDa
shipped in
wet ice
storage temp.
−20°C
General description
Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.
Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.
Application
Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.. Product P7640 has been used to study the monoclonal alkaline phosphatase-anti-alkaline phosphatase (APAAP) complex[1].
High specific activity grade recommended for antibody and protein conjugation.
Biochem/physiol Actions
Alkaline phosphatase, from bovine intestinal mucosa, is most stable in the pH range 7.5-9.5. The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols and it requires zinc, and magnesium or calcium divalent ions for activity.
The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.
Analysis Note
Package sizes are based on DEA units
Other Notes
One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)
1 of 1
This Item | |||
|---|---|---|---|
| biological source bovine intestinal mucosa | biological source - | biological source bovine intestinal mucosa | biological source - |
| specific activity ≥10 DEA units/mg solid | specific activity ≥5,500 DEA units/mg protein | specific activity ≥2,000 DEA units/mg protein | specific activity ≥4,000 DEA units/mg protein |
| form lyophilized powder | form (Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6) | form buffered aqueous solution | form buffered aqueous glycerol solution |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mol wt dimer ~160 kDa | mol wt dimer ~160 kDa | mol wt dimer ~160 kDa | mol wt dimer ~160 kDa |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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