P3644

L-α-Phosphatidylcholine

from soybean, Type IV-S, ≥30% (enzymatic)

• Synonym(s): 1,2-Diacyl-sn-glycero-3-phosphocholine, 3-sn-Phosphatidylcholine, L-α-Lecithin, Azolectin, PC
• CAS Number: 8002-43-5
• Beilstein: 5209585
• EC Number: 232-307-2
• UNSPSC Code: 51321705
• NACRES: NA.25

Properties

• biological source: soybean
• type: Type IV-S
• form: solid
• concentration: ≥30% (enzymatic)
• solubility: chloroform: soluble 100 mg/mL, clear to slightly hazy, yellow to orange
• functional group: phospholipid
• lipid type: phosphoglycerides
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C42H80NO8P/c1-6-8-10-12-14-16-18-20-21-23-25-27-29-31-33-35-42(45)51-40(39-50-52(46,47)49-37-36-43(3,4)5)38-48-41(44)34-32-30-28-26-24-22-19-17-15-13-11-9-7-2/h14,16,20-21,40H,6-13,15,17-19,22-39H2,1-5H3/b16-14-,21-20-/t40-/m1/s1
• InChI key: JLPULHDHAOZNQI-ZTIMHPMXSA-N

Description

Application

L-α-Phosphatidylcholine is suitable for use:

• as a component of human erythroid massive amplification culture (HEMA^def) medium for clinical expansion of human erythroblasts
• in the chloride efflux assay to examine the chloride permeability of lipid vesicles conferred by soluble CLIC1
• in an assay to measure solubilized wax synthase activity
• as a component of buffer A used for the resuspension of the cholate-solubilized catalytic unit of adenylate cyclase
• as a cryoprotectant for freezing ruminant sperm
• as a substrate for the estimation of phospholipase activity
• to make liposomes to assess their efficiency of recruitment of ARF (ADP ribosylation factor) and AP-1 (adaptor protein)
• in the preparation of the isolated catalytic unit of soluble adenylate cyclase

Biochem/physiol Actions

Phosphatidylcholine is the major membrane phospholipid in eukaryotic cells, forming the structural component of these membranes. It also serves as a reservoir for several lipid messengers and a source for several bioactive lipids such as, lysophosphatidylcholine, phosphatidic acid, diacylglycerol, lysophosphatidylcholine, platelet activating factor, and arachidonic acid.

A major structural phospholipid in brain, comprising approx. 15% of total lipid; primarily localized to gray matter.

Preparation Note

Purified from P 5638

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves