P1868

Protein Phosphatase 2A₂ from bovine kidney

buffered aqueous glycerol solution

• Synonym(s): PP2A₂
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bovine kidney
• Quality Level: 200
• form: buffered aqueous glycerol solution
• specific activity: ~2.0 U/vial
• mol wt: dimer 101 kDa (65 kDa and 36 kDa)
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Protein phosphatase 2A (PP2A) is a specific protamine-kinase-inactivating phosphatase, one common physiological form of which is PP2A₂.

Application

Protein phosphatase 2A₂ has been used in a study to investigate two heat-stable protein inhibitors. It has also been used in a study to describe the purification and properties of a protamine kinase from bovine kidney microsomes.

Biochem/physiol Actions

Protein Phosphatase 2A2 from bovine kidney was shown to be a unique inhibitor of protamine kinase while other phosphatases in the same family including PP1, PP2B, PP2C did not show any inhibition.

Unit Definition

One unit will release 1 nanomole of inorganic phosphate from (32)P-labeled phosphorylase per minute at pH 7.0 at 30 deg C.

Physical form

Solution of 1 μg per vial in 50 μl of 50 mM Tris-HCl, pH 7.0, containing 14 mM β-Mercaptoethanol, 1mM benzamidine, 0.1 mM PMSF, 1mM EDTA, and 50% glycerol.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable