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M6770

MCDB 201 Medium

With trace elements, L-glutamine and 30 mM HEPES, powder, suitable for cell culture

Synonym(s):

MCDB medium

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About This Item

NACRES:
NA.75
UNSPSC Code:
41161501
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form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: 7.149 g/L (30mM)
glucose: 1.441 g/L (Dextro)
phenol red: 0.001242 g/L
sodium pyruvate: 0.055 g/L
L-glutamine: 0.14615 g/L

shipped in

ambient

storage temp.

2-8°C

General description

MCDB 201 medium is a modified Ham′s nutrient mixture F-12 that is synthesized for the clonal growth of chicken embryo fibroblasts.

Application

MCDB 201 Medium has been used as a component of:
  • the hepatic differentiation medium to culture pluripotent stem cells,
  • epididymal growth medium for culturing of preadipocytes,
  • Dulbecco′s Modified Eagle Medium (DMEM)/F12 for culturing of human umbilical cord mesenchymal stem cells

Preparation Note

Formulated to contain 17.7 grams of powder per liter of medium.

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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