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MOPS-EDTA-Sodium Acetate (MESA)

Name: MOPS-EDTA-Sodium Acetate (MESA)
Brand: SIGMA

powder for RNA electrophoresis buffers

Synonym(s):

MESA Buffer

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About This Item

NACRES:

NA.25

UNSPSC Code:

12161700

Pricing and availability is not currently available.

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Properties

grade

Molecular Biology

Quality Segment

200

product line

BioReagent

form

powder

technique(s)

electrophoresis: suitable

pH

6.8-7.2(reconstituted per directions)

solubility

H₂O: soluble, faintly yellow

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

room temp

Description

General description

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Application

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

Preparation Note

1x MESA buffer contains:

40 mM MOPS
10 mM sodium acetate
1 mM EDTA (pH 8.3)

Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

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This Item	T8280	T7527	PPB008
Sigma-Aldrich M5755 MOPS-EDTA-Sodium Acetate (MESA)	Sigma-Aldrich T8280 Tris Acetate-EDTA buffer	Sigma-Aldrich T7527 Tris-Borate-EDTA buffer	Sigma-Aldrich PPB008 Tris Acetate-EDTA buffer
description powder for RNA electrophoresis buffers	description 10× concentrate, BioReagent, Molecular Biology, DNase and RNase, none detected, powder blend, suitable for electrophoresis	description BioReagent, Molecular Biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis	description pH 8.3, pHast Pack^™, powder
grade Molecular Biology	grade Molecular Biology	grade Molecular Biology	grade -
technique(s) electrophoresis: suitable	technique(s) -	technique(s) -	technique(s) -
product line BioReagent	product line BioReagent	product line BioReagent	product line pHast Pack^™
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level -
form powder	form powder blend	form powder blend	form powder
pH 6.8-7.2(reconstituted per directions)	pH 8.1-8.5	pH 8.1-8.5 (5 ×)	pH 8.3

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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