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PNGase F Glycosidase

Name: PNGase F Glycosidase
Brand: SIGMA

Removes N-linked glycans, suitable for mass spectrometry, from Elizabethkingia miricola

Synonym(s):

N-Glycosidase F, Peptide N-glycosidase

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About This Item

CAS Number:

83534-39-8

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

3.5.1.52 (BRENDA, IUBMB)

MDL number:

MFCD06656235

Specific activity:

≥20000 units/mg protein

Biological source:

bacterial (Elizabethkingia miricola)

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Properties

Product Name

PNGase F from Elizabethkingia miricola, buffered aqueous solution

biological source

bacterial (Elizabethkingia miricola)

Quality Segment

200

conjugate

(N-linked)

form

buffered aqueous solution

specific activity

≥20000 units/mg protein

mol wt

36 kDa

shipped in

wet ice

storage temp.

2-8°C

Description

General description

PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides.

Application

PNGase F from Elizabethkingia miricola has been used for the deglycosylation of the proteins.

Used to deglycosylate protein.

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Physical form

Supplied as a solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTA

Other Notes

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

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This Item	P9120	362185	G0535
Sigma-Aldrich G5166 PNGase F from Elizabethkingia miricola	Sigma-Aldrich P9120 PNGase F from Elizabethkingia meningoseptica	Sigma-Aldrich 362185 N-Glycosidase F, Elizabethkingia meningosepticum	Sigma-Aldrich G0535 Glycopeptidase A from almonds
description buffered aqueous solution	description recombinant, expressed in E. coli, set of 100 units nanomolar unit	description -	description buffered aqueous glycerol solution, ≥0.05 unit/mL
specific activity ≥20000 units/mg protein	specific activity ≥10 units/mg protein	specific activity ≥20,000 units/mg protein, ≥4500 units/mL	specific activity -
biological source bacterial (Elizabethkingia miricola)	biological source -	biological source bacterial (Elizabethkingia meningosepticum)	biological source -
form buffered aqueous solution	form -	form liquid	form buffered aqueous glycerol solution
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in -
mol wt 36 kDa	mol wt 36 kDa	mol wt -	mol wt -

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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