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F1058

FSL-Fluorescein

Synonym(s):

FSL-FLRO4(fluorescein)-SA2-L1

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About This Item

Empirical Formula (Hill Notation):
C73H109N4O15PS
Molecular Weight:
1345.70
UNSPSC Code:
41116100
PubChem Substance ID:
329799672
NACRES:
NA.32
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Quality Segment

storage temp.

−20°C

SMILES string

CCCCCCCC\C=C/CCCCCCCC(=O)OCC(COP(O)(=O)OCCNC(=O)CCCCC(=O)NCCCCCNC(=S)Nc1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35)OC(=O)CCCCCCC\C=C/CCCCCCCC

InChI

1S/C73H109N4O15PS/c1-3-5-7-9-11-13-15-17-19-21-23-25-27-29-32-40-69(82)87-55-60(90-70(83)41-33-30-28-26-24-22-20-18-16-14-12-10-8-6-4-2)56-89-93(85,86)88-51-50-75-68(81)39-35-34-38-67(80)74-48-36-31-37-49-76-72(94)77-57-42-45-62-61(52-57)71(84)92-73(62)63-46-43-58(78)53-65(63)91-66-54-59(79)44-47-64(66)73/h17-20,42-47,52-54,60,78-79H,3-16,21-41,48-51,55-56H2,1-2H3,(H,74,80)(H,75,81)(H,85,86)(H2,76,77,94)/b19-17-,20-18-

InChI key

OZXSVOLFZKVFHP-CLFAGFIQSA-N

Application

FSL Constructs Video Protocol
All FSL constructs disperse in biocompatible media and spontaneously and stably incorporate into cell membranes. Cells modified with FSL constructs are known as kodecytes and usually maintain their normal vitality and functionality. FSL-fluorescein has been specifically designed to create fluorescein-labeled live cells (kodecytes) but can also be used to modify other hydrophobic surfaces including fixed cells, virions, and solid phase surfaces. Fluorescein-labeled kodecytes can be visualized at the approximate absorbance wavelength of 488 nm and emission at 518 nm.
All FSL constructs disperse in biocompatible media and spontaneously and stably incorporate into cell membranes. FSL-fluorescein has been specifically designed to create fluorescein-labeled live cells (kodecytes) but can also be used to modify other hydrophobic surfaces including fixed cells, virions, and solid phase surfaces.

Other Notes

FSL-fluorescein is a KODE™ technology construct designed to label hydrophobic surfaces including living cells with biotin. All FSL constructs consist of three essential designable features:
  • functional component (F)
  • spacer (S)
  • diacyl lipid (L)
FSL-fluorescein is comprised of a fluorescein residue representing F, conjugated via a spacer (SA2) to an an activated adipate derivative of dioleoylphosphatidylethanolamine (L1).
Packaged as 1 mg FSL-Fluorescein with 0.42 mg sodium bicarbonate to improve solubility.

Legal Information

KODE Constructs are supplied by KODE Biotech Materials Limited under license from KODE Biotech Limited.
KODE is a trademark of KODE Biotech Ltd.

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This Item
F930746939F9682
FSL-Fluorescein

Sigma-Aldrich

F1058

FSL-Fluorescein

FSL-A(tri)

Sigma-Aldrich

F9307

FSL-A(tri)

Fluorescein diacetate 5-maleimide suitable for fluorescence

Sigma-Aldrich

46939

Fluorescein diacetate 5-maleimide

FSL-LEa(tri)

Sigma-Aldrich

F9682

FSL-LEa(tri)

description

-

description

-

description

suitable for fluorescence

description

-

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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Questions

1–6 of 6 Questions  

  1. Will the product F1058, FSL-Fluorescein label dead cells?

    1 answer

    1. The product F1058, FSL-Fluorescein, is designed to insert into the membranes of live cells. But it can also be used to label fixed cells, enveloped virions, and solid phase surfaces.

      Helpful?

  2. How many cells can I label with product F1058, FSL-Fluorescein?

    1 answer

    1. The exact number of cells may vary depending on cell type and cell condition. The recommended concentration to use for labeling is 10-100ug/ml (~7.4 mM-74mM), which is mixed in a 1:1 volume with cells.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. What concentration of F1058, FSL-Fluorescein do I use to label cells?

    1 answer

    1. For cell labeling we recommend using a concentration of 10-100ug/ml of F1058, FSL-Fluorescein diluted in PBS. Label cells with FSL-Fluorescein in PBS at a 1:1 volume to volume ratio. The exact concentration may need to be optimized for the cells and the application. Keep incubations in the dark.

      Helpful?

  5. May I use serum with the product F1058, FSL-Fluorescein?

    1 answer

    1. We do not recommend diluting the product F1058, FSL-Fluorescein, in lipid-containing buffers. The FSL will associate with the lipids and reduce the efficiency of insertion into cell membranes.

      Helpful?

  6. What should I use to reconstitute product F1058, FSL-Fluorescein?

    1 answer

    1. We recommend reconstituting 1 mg of product F1058, FSL-Fluorescein with 0.5ml of saline or PBS for a concentration of 2 mg/ml.

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