E2039

Chondroitinase AC from Flavobacterium heparinum

recombinant, expressed in E. coli, ≥200 units/mg protein, For Chondroitin Sulfate Analysis

• Synonym(s): Chondroitin AC lyase
• CAS Number: 9047-57-8
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 4.2.2.5 (BRENDA, IUBMB)
• MDL number: MFCD00130796
• Specific activity: ≥200 units/mg protein
• Assay: ≥90% (SDS-PAGE)
• Recombinant: expressed in E. coli

Properties

• recombinant: expressed in E. coli
• Quality Segment: 200
• conjugate: (Glucosaminoglycan)
• assay: ≥90% (SDS-PAGE)
• form: lyophilized solid
• specific activity: ≥200 units/mg protein
• shipped in: dry ice
• storage temp.: −20°C

Description

Application

Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).

Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.

Highly purified to remove interferring β-glucuronidase and protease activities for use in the hydrolysis of chondroitin sulfate pror to HPLC analysis.

Other Notes

1 unit is defined as the amount of enzyme that will liberate 1.0 μmole per minute of unsaturated disaccharides from chondroitin sulfate A at pH 6.7 at 37 °C as measured by the change in A232. The ε^μΜ for the reaction product Δ-Di-4S (chondroitin sulfates A and B) is 5.1 and 5.5 for Δ-Di-6S (chondroitin sulfate C).

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable