If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
D8417
4′,6-Diamidino-2-phenylindole dihydrochloride
powder, BioReagent, suitable for cell culture, ≥98% (HPLC), suitable for fluorescence
Synonym(s):
2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride
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About This Item
product line
BioReagent
Quality Segment
assay
≥98% (HPLC)
form
powder
technique(s)
cell culture | mammalian: suitable
solubility
H2O: 20 mg/mL, PBS: insoluble
ε (extinction coefficient)
30 at 263 nm in H2O
fluorescence
λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
suitability
suitable for fluorescence
storage temp.
2-8°C
SMILES string
Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChI key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
General description
Application
Biochem/physiol Actions
Disclaimer
1 of 1
This Item | |||
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| description powder, BioReagent, suitable for cell culture, ≥98% (HPLC), suitable for fluorescence | description For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL | description for nucleic acid staining | description suitable for fluorescence, BioReagent, ≥95.0% (HPLC) |
| suitability suitable for fluorescence | suitability suitable for nucleic acid staining | suitability suitable for fluorescence | suitability suitable for fluorescence |
| technique(s) cell culture | mammalian: suitable | technique(s) transfection: suitable | technique(s) transfection: suitable | technique(s) - |
| fluorescence λex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 340 nm; λem 488 nm (nur DAPI) | fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) | fluorescence λex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 340 nm; λem 488 nm (nur DAPI) | fluorescence λex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA, λex 340 nm; λem 488 nm (nur DAPI) |
| solubility PBS: insoluble, H2O: 20 mg/mL | solubility water: soluble | solubility PBS: insoluble, H2O: 20 mg/mL | solubility DMF: soluble, H2O: soluble |
| form powder | form liquid | form powder | form powder |
| assay ≥98% (HPLC) | assay - | assay ≥98% (HPLC) | assay ≥95.0% (HPLC) |
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Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
Target Organs
Respiratory system
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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