CHSE-214 Cell Line from salmon
91041114, fish embryo, Not specified
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About This Item
UNSPSC Code:
41106514
product name
CHSE-214 Cell Line from salmon, 91041114, embryo
biological source
fish embryo
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Salmon embryo
Cell Line Description
Derived from a Chinook salmon (Oncorhynchus tshawytscha) embryo, susceptible to a wide range of fish viruses and in many instances replicate high titres.
Application
CHSE-214 cell line has been used for the growth and titration of infectious pancreatic necrosis virus (IPNV) from samples. It has also been used to carry out cell infection assays to determine the effect of tropodithietic acid (TDA)-exposed isolates.
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6; i.e seeding at 1-4 x 10,000 cells per cm using 0.25% trypsin or trypsin/EDTA, cells detach after 5-10 minutes at room temperature. Grow cells in dark (e.g. wrapped in foil); 5% CO2; 21°C. Freeze cells in 5% DMSO and 95% foetal bovine serum (FBS). Fish cell lines detach easily during transit if the culture is too young. For resuscitation seed flasks at 4-5 x 10,000 cells per cm and then split cells 1:3 to 1:4 and culture for at least one week with 1-2 media changes before shipping.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
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