HeB5

11033104, mouse liver, Epithelial

• Synonym(s): Hepa-1c1c7
• UNSPSC Code: 41106514

Properties

• Product Name: HeB5, 11033104
• biological source: mouse liver
• packaging: tube of 5 μg 11033104-DNA-5UG; pkg of vial of cells 11033104-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice

Description

Cell Line Origin

Hepa-1c1c7, genetic modification, Xenobiotic-Responsive Element (XRE)

Cell Line Description

HeB5 is a derivative of Hepa-1c1c7 (Sigma product number 95090613) cell line stably transfected with a firefly luciferase reporter construct bearing Xenobiotic-Responsive Element (XRE) driven by a TATA promoter. HeB5 can be used for detection of mouse CYP1A induction by chemicals.

Culture Medium

Alpha-MEM + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 16μg/ml Blasticidin S HCL.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10, i.e. seeding at 2 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.