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C7886

Sigma-Aldrich

Creatine Phosphokinase from bovine heart

Type III, salt-free, lyophilized powder, ≥30 units/mg protein

Synonym(s):

ATP: Creatine N-Phosphotransferase, CPK, Creatine Kinase, Phosphocreatine phosphokinase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

biological source

bovine heart

description

Contains primarily isozyme MM

type

Type III

form

salt-free, lyophilized powder

specific activity

≥30 units/mg protein

mol wt

80 kDa

storage temp.

−20°C

General description

Research area: Cell Signaling
Creatine phosphokinase (CPK) is an intracellular enzyme found predominantly in skeletal muscle, brain, and myocardium. CPK consists of two polypeptide subunits, namely M (muscle type) and B (brain type). The lighter subunit is present in larger amounts. The genes encoding these subunits are on distinct chromosomes, with B on 14q32 and M on 19q13. These subunits facilitate the development of three tissue-specific isoenzymes: CPK-MB (cardiac muscle), CPK-MM (skeletal muscle), and CPK-BB (brain).

Application

Creatine Phosphokinase from bovine heart has been used:

  • in the reaction mix for the import of in vitro synthesized wild-type FLAG/MYC-tagged LYR Motif Containing 7 (LYRM7-F/M) or the LFK tripeptide replaced with alanine (LFK-AAA) mutant into isolated mitochondria
  • in autoubiquitination assay
  • in the preparation of premix buffer for adenylyl cyclase activity
  • in the preparation of E-mix for in vitro nuclear assembly and isolation
  • in in vitro vesicular fusion assay.
  • in ATP regeneration system, to facilitate the reactivation of nonmotile axonemes.
  • in subcellular in vitro fusion assay of autophagosome with lysosome.
  • in in vitro protein translation assay.
  • for tATPase assay of myofibrillar protein isolated from rabbit. This assay evaluated the kinetic influence of bound creatine kinase (CK) on Ca2+-activated myosin ATPase.
  • for the enzymatic hydrolysis of protein samples during tryptophan estimation by pyrolysis gas chromatography.
Creatine phosphokinase from bovine heart has been used to investigate whether endothelial cell growth is stimulated by ischemic hearts. Creatine phosphokinase from bovine heart has also been used to evaluate the effect of high but nontoxic dietary intake of copper and selenium on metabolism in calves.

Biochem/physiol Actions

Creatine kinase plays a key role in the energy metabolism of cells with intermittently high and fluctuating energy requirements. Examples of such cells include cardiac or skeletal muscle cells and neural tissues of brain and retina. The enzyme catalyzes the reversible transfer of the phosphoryl group from phosphorylcreatine to ADP, in order to generate ATP. The molecular mass of the protein is found to be approximately 80,000 Da. It is made up of 2 subunits, each having a molecular weight of 40,000 ± 2000. The lighter subunit is present in larger amounts.CPK is released from the cellular cytosol into the systemic circulation due to disruption of cell membranes caused by hypoxia or other injuries. Furthermore, it serves as a diagnostic indicator for rhabdomyolysis and acute myocardial infarction (AMI), along with other medical disorders such as acute muscle injury, arrhythmias, and congestive cardiac failure.

Caution

The use of 0.1% albumin in the reaction buffer is recommended to avoid inactivation due to dilution.

Unit Definition

One unit will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30 °C.

Analysis Note

Protein determined by biuret.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A general strategy for discovery of inhibitors and activators of RING and U-box E3 ligases with ubiquitin variants
Gabrielsen M, et al.
Molecular Cell, 68(2), 456-470 (2017)
Creatine Kinase
Cabaniss CD, et al.
Clinical Methods: The History, Physical, and Laboratory Examinations, 3 (1990)
A C Galloway et al.
Surgery, 96(2), 435-439 (1984-08-01)
The development of myocardial ischemia is known to elicit the formation and enlargement of collateral vessels. The stimulus for these events is unknown. We have investigated the possibility that cardiac tissue releases a factor that can stimulate endothelial cell proliferation.
Cholesterol impairs autophagy-mediated clearance of amyloid beta while promoting its secretion
Barbero-Camps E, et al.
Autophagy, 14(7), 1129?1154-1129?1154 (2018)
S M Krause et al.
The Journal of biological chemistry, 267(4), 2480-2486 (1992-02-05)
The kinetic influence of bound creatine kinase (CK) on the Ca(2+)-activated myosin ATPase was evaluated. ATPase rates were measured from 0.8 microM to 3.2 mM MgATP. Under control conditions, the apparent KmATP was 79.9 +/- 13.3 microM. In contrast, the

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