C3138

Cathepsin D from bovine spleen

lyophilized powder, ≥2.0 units/mg protein

• Synonym(s): CTSD
• CAS Number: 9025-26-7
• UNSPSC Code: 12352204
• NACRES: NA.32
• EC Number: 3.4.23.5 (BRENDA, IUBMB)
• MDL number: MFCD00130749
• Specific activity: ≥2.0 units/mg protein
• Assay: 10—70% protein (biuret)
• Biological source: bovine spleen

Properties

• biological source: bovine spleen
• Quality Segment: 200
• assay: 10—70% protein (biuret)
• form: lyophilized powder
• specific activity: ≥2.0 units/mg protein
• mol wt: ~45 kDa
• manufacturer/tradename: Sigma-Aldrich
• technique(s): activity assay: suitable
• color: dark brown
• suitability: suitable for molecular biology
• UniProt accession no.: P80209
• application(s): life science and biopharma
• storage temp.: −20°C
• Gene Information: cow ... CTSD(282883)

Description

General description

Research area: Cell Signaling

Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region.

Application

Cathepsin D from bovine spleen has been used:

• in in vitro dose-dependent fluorometric activity assays.
• in in vitro myelin oligodendrocyte glycoprotein (MOG) digestion to study the uptake of malondialdehyde (MDA)-modified MOG and its implications in central nervous system autoimmunity.
• for enzymatic digestion of the proteoglycan moiety of the articular cartilage in order to determine its dynamic elastic modulus at two different levels of tissue organization.
• in cathepsin D activity assay.

Biochem/physiol Actions

Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.

Endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts.

Physical form

Lyophilized powder containing citrate buffer salts

Other Notes

One unit will produce an increase in A₂₈₀ of 1.0 per min per mL at pH 3.0 at 37 °C measured as TCA-soluble products using hemoglobin as substrate (1 cm light path).

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable