C0897
Citrate Lyase from Klebsiella pneumoniae
lyophilized powder, ≥0.20 unit/mg solid
Synonym(s):
Citrate oxaloacetate lyase
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About This Item
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form
lyophilized powder
Quality Level
specific activity
≥0.20 unit/mg solid
mol wt
>5 kDa
storage temp.
2-8°C
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Application
Citrate Lyase from Klebsiella pneumoniae has been used in the digestion of low molecular weight human milk fraction (>5 kDa referred to as 5kF).
Biochem/physiol Actions
Citrate lyase is found in several microorganisms and catalyzes the first step of citrate degradation, forming acetate and oxaloacetate. The enzyme contains 3 polypeptide subunits, α-subunit (a transferase), β-subunit (acyl lyase) and γ-subunit (acyl-carrier protein).
Unit Definition
One unit will convert 1.0 μmole of citrate to oxalacetate per min at pH 7.6 at 25 °C.
Physical form
Lyophilized powder containing bovine serum albumin, sucrose, and MgSO4
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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M Hupperich et al.
European journal of biochemistry, 192(1), 161-166 (1990-08-28)
The primary structure of the beta-subunit (acyl lyase subunit) of citrate lyase from Klebsiella pneumoniae (ATCC 13,882) was determined with protein chemical methods. The polypeptide chain consists of 289 amino acid residues and has a molecular mass of 31,352 Da.
Djamel Drider et al.
Genetics and molecular research : GMR, 3(2), 273-281 (2004-07-22)
Citrate is present in many natural substrates, such as milk, vegetables and fruits, and its metabolism by lactic acid bacteria (LAB) plays an important role in food fermentation. The industrial importance of LAB stems mainly from their ability to convert
M Martín et al.
FEMS microbiology letters, 174(2), 231-238 (1999-05-26)
The citMCDEFGRP cluster from Leuconostoc paramesenteroides involved in citrate utilization was cloned and its nucleotide sequence determined. Homology of the inferred gene products with characterized enzymes reveals that citP encodes the citrate permease P, citC the citrate ligase and citDEF
S Bekal-Si Ali et al.
Journal of bacteriology, 181(14), 4411-4416 (1999-07-10)
In this paper, we describe two open reading frames coding for a NAD-dependent malic enzyme (mae) and a putative regulatory protein (clyR) found in the upstream region of citCDEFG of Leuconostoc mesenteroides subsp. cremoris 195. The transcriptional analysis of the
M Isabel Burguete et al.
Dalton transactions (Cambridge, England : 2003), (36)(36), 4027-4033 (2007-09-11)
A turn-on fluorescent indicator for citric acid (citrate) has been developed, displaying high emission enhancement (+1500%) and low interference by other carboxylates. The sensor is based on the non-emissive copper(II) complex of a fluorescent amino amide, which, upon addition of
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