The properties and content of this product have not been determined. The percentage of protein is lot specific. The Certificate of Analysis (CoA) provides information on the quality control release tests.
B4882
Bromelain from pineapple stem
≥3 units/mg protein
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-387-5
MDL number:
EC Number:
Specific activity:
≥3 units/mg protein
Pricing and availability is not currently available.
form
lyophilized powder
Quality Segment
specific activity
≥3 units/mg protein
composition
Protein, ≥30% biuret
storage temp.
−20°C
Application
Bromelain from pineapple stem has been used:
- to evaluate the aqueous micellar two-phase systems composed of ionic liquids as co-surfactants
- as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) and in cells proliferation assay
- to study its potential analgesic effect and to evaluate its hydrolyzingeffects on proenkephalin (PENK)-derived peptides containing enkephalin sequences
Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen .
Biochem/physiol Actions
Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product B4882 is from pineapple stem. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.
Preparation Note
The product may be suspended in acetate buffer, pH 4.5 at 1 mg/mL concentration, yielding a hazy, off-white suspension.
Other Notes
One unit will release 1.0 micromole of p-nitrophenol from N-alpha-CBZ-L-Lysine p-nitrophenyl ester per minute at pH 4.6 at 25 °C. One old titrimetric unit (pH 4.5 at 45 °C) is equivalent to approximately 1.7 new units (pH 4.6 at 25 °C).
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This Item | |||
|---|---|---|---|
| description ≥3 units/mg protein | description lyophilized powder, ≥100 units/mg protein | description lyophilized powder, ≥100 units/mg protein | description lyophilized powder, ≥3 units/mg protein |
| specific activity ≥3 units/mg protein | specific activity ≥100 units/mg protein | specific activity ≥100 units/mg protein | specific activity ≥3 units/mg protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| composition Protein, ≥30% biuret | composition Protein, ≥20% Lowry | composition Protein, 20-74% Lowry | composition Protein, ~70% biuret |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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I WILL USE BROMELAIN (B4882-10G ).IN A RESEARCH PROJECT. BROMELAIN IS COMPOSED OF MANY ENZYMES. THEY WERE ASKED WHAT the PROPERTIES AND CONTENT OF THE BROMELAIN YOU USE. I WOULD BE HAPPY IF YOU HELPED ME WITH
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