The production process for A7641 does not involve the use of tris. All pH adjustments and chromatography steps are carried out using Acetic acid or Ammonium hydroxide. The material is essentially free of salts before undergoing lyophilization, although there is a possibility of trace amounts of NaCl due to chromatography. The NaCl concentration would be less than 0.01%.
A7641
Albumin from chicken egg white
lyophilized powder
Synonym(s):
Albumin from hen egg white, Ovalbumin
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352202
eCl@ss:
42010201
NACRES:
NA.25
Pricing and availability is not currently available.
Recommended Products
biological source
chicken egg white
Quality Level
type
Grade VII
Assay
≥95% (agarose gel electrophoresis)
form
lyophilized powder
mol wt
44287 Da by calculation
purified by
chromatography
technique(s)
bioconjugation: suitable
UniProt accession no.
storage temp.
2-8°C
Gene Information
chicken ... OVAL(396058)
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General description
Chicken egg albumin is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylated-glycoprotein. From the amino acid sequence, the peptide portion of the molecule consists of 385 residues and has a molecular weight of 42.7 kDa. [1]
Ovalbumin, an egg white protein is produced in the oviduct of hen. It has 3 sites of post synthetic modification and an N-terminal acetyl group.[2] This product is free of S-ovalbumin, a heat stable form of egg albumin formed when eggs are stored.
Application
A phosphorylated glycoprotein which is useful as a marker (~45 kDa) for protein electrophoresis. Has been used to produce an animal model of asthma.
Albumin from chicken egg white has been used:
- as a component in reaction buffer for in vitro enzymatic assay[3]
- to simulate dangerous protein toxins[4]
- as a component in marker solution[5]
The product is useful as a marker (∼45 kDa) for protein electrophoresis. It has been used to produce an animal model of asthma.
Ovalbumin was used as medium for cell incubation in EliCell assay for the detection of released cytokines from eosinophil.[6]
It was also used in a study testing intestinal macromolecule absorption in fetal pig after infusion of colostrum in utero.[7]
Ovalbumin was used as medium for cell incubation in EliCell assay for the detection of released cytokines from eosinophil.[6]
It was also used in a study testing intestinal macromolecule absorption in fetal pig after infusion of colostrum in utero.[7]
Biochem/physiol Actions
Differential scanning calorimetry indicates that chicken egg albumin denatures at 84 °C.[8] Ovalbumin can be used as a carrier protein to conjugate to synthetic peptides for use as an immunogen.
Ovalbumin is preferred as a nutrient supplement. It can serve as a drug carrier. It possess tumor necrosis releasing factors, that can play a great role in tumor suppression.[2]
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1A
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Articles
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
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Is there any information available regarding whether chicken egg white albumin product A7641 was prepared in a buffer solution containing tris prior to lyophilization, or if it was prepared from a pure form of chicken egg white albumin without any buffer?
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