Unfortunately, a purification step to remove cell wall polysaccharide from peptidoglycan is not performed.
77140
Peptidoglycan from Staphylococcus aureus
cell wall component
Synonym(s):
Peptidoglycan from S. aureus
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About This Item
Pricing and availability is not currently available.
Quality Level
form
lyophilized powder
solubility
soluble, hazy to turbid, colorless to faintly yellow
storage temp.
−20°C
Application
Peptidoglycan from Staphylococcus aureus has been used:
- for the stimulation of lymphocytes: peptidoglycan activates the toll-like receptor 2 (TLR2), present in mammalian cells. It works as an antagonist of Poly (I:C)[1]
- for g-type lysozyme stimulation by intraperitoneally inoculating it into sole specimens
- in phosphate buffer saline and injected into Labeo rohita fingerlings, to study its effect on nucleotide-binding oligomerization domain-2 (NOD-2) gene expression
Biochem/physiol Actions
The cell wall of gram positive bacteria is largely made up of peptidoglycan. It is composed of polysaccharide chains interlinked by peptide bridges. This peptidoglycan gives a certain rigidity to the cell wall and gives the cell mechanical strength. It is a surface molecule which is known to stimulate immune reactions including cytokine production to fever.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
Major component of the cell wall in gram positive organisms. Consists of a glycan backbone with alternating β 1-4 linked residues of N-acetyl-D-glucosamine and muramic acid. The immunological activities of bacterial peptidoglycans; Peptidoglycan types of bacterial cell walls and their taxonomic implications; Antibody response to peptidoglycan during staphylococcal infections.
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This Item | |||
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| solubility soluble, hazy to turbid, colorless to faintly yellow | solubility - | solubility 10 mM HCl: soluble 1.0 mg/mL, clear to faintly turbid, colorless | solubility H2O: soluble 1.9-2.1 mg/mL, clear, colorless to faintly yellow |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form powder | form powder | form essentially salt-free, lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
Instructions
L J Wheat et al.
The Journal of infectious diseases, 147(1), 16-22 (1983-01-01)
Levels of antibodies to peptidoglycan were measured by solid-phase radioimmunoassay in 76 patients with Staphylococcus aureus infections and 44 patients with infections caused by other bacteria. Levels of IgM antibodies to peptidoglycan were elevated in 24% of the patients with
Genomic characterization, phylogeny and gene regulation of g-type lysozyme in sole (Solea senegalensis)
Ponce M, et al.
Fish & Shellfish Immunology, 31(6), 925-937 (2011)
The immunological activities of bacterial peptidoglycans.
D E Stewart-Tull
Annual review of microbiology, 34, 311-340 (1980-01-01)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 77140-25MG | 04061833267264 |
| 77140-10MG | 04061832925233 |
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Are there any purification steps to remove cell wall polysaccharide from peptidoglycan? Thanks!
1 answer-
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In our research, we are using PGN-Sa from Sigma; I would like to know the exact chemical composition of this product, both the glycan part and the peptide stem.
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Unfortunately, the exact chemical composition of this product is not available.
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When I dissolve the 10mg PGN in 10mL of ultrapure water, there are white chunks that float to the bottom. Are there any recommendations for making these particles more uniform in the suspension? Or a better way to dissolve the PGN?
1 answer-
This product is not tested for solubility. Peptidoglycans are only minimally soluble and will often produce a hazy or turbid solution. Sonication and/or further dilution may improve the solubility.
The product issue you are reporting should be investigated by our Technical Service team, who can assist you with further troubleshooting and/or investigation. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice and click on "Report Product Issues" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
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How can solutions of Product 77140, Peptidoglycan, be stored?
1 answer-
Sigma has not evaluated the solution stability of solutions. It is therefore recommended that fresh solutions be prepared.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product 77140, Peptidoglycan, be solubilized in water?
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No, the product is not soluble in water, or in other solvents for that matter. It forms a suspension in water.
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Does Product 77140, Peptidoglycan, have a recommended retest date?
1 answer-
If a product has a recommended retest date this would be on the Certificate of Analysis found at our website. If no recommended retest date is found on the Certificate of Analysis, then the guarantee of quality is one year from the time of delivery for unopened bottles stored at the recommended temperature.
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