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Lipase from Aspergillus oryzae

Name: Lipase from <I>Aspergillus oryzae</I>
Brand: SIGMA

lyophilized, powder, white, ~50 U/mg

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About This Item

CAS Number:

9001-62-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-619-9

EC Number:

3.1.1.3 (BRENDA, IUBMB)

MDL number:

MFCD00131509

Specific activity:

~50 U/mg

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Properties

form

powder

Quality Segment

100

quality

lyophilized

specific activity

~50 U/mg

color

white

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

Description

General description

Lipase is a carboxylesterase, which belongs to the α/β hydrolase fold family. It consists of a lid domain, hinge domain, and a catalytic triad Ser-His-Asp/Glu. Aspergillus oryzae lipase has a three-dimensional structure is an α/β hydrolase fold similar to that of the esterase enzyme.

Application

Lipase from Aspergillus oryzae has been used as a component of mixed enzyme solution to determine the viable cell numbers in the biofilms formed by Listeria monocytogenes strains.

Biochem/physiol Actions

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids. Aspergillus oryzae lipase might be used in the production of biodiesel from waste cooking oil. It is used in the acetylation of cellulose and ochratoxin A degradation, and asymmetric organic synthesis. Lipase from Aspergillus oryzae is an industrial enzyme that has various uses in food, detergent, and pharmaceutical industries. It is useful in immobilization studies for 1,3-dioleoyl-2-palmitoylglycerol (OPO) synthesis.

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Other Notes

1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40°C (triolein, Cat. No. 62314 as substrate)

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1 of 1

This Item	L4277	L0777	62301
Sigma-Aldrich 62285 Lipase from Aspergillus oryzae	Sigma-Aldrich L4277 Lipase from Aspergillus oryzae	Sigma-Aldrich L0777 Lipase from Aspergillus oryzae	Sigma-Aldrich 62301 Lipase from Aspergillus niger
description lyophilized, powder, white, ~50 U/mg	description ≥20,000 U/g	description solution, ≥100,000 U/g, white, beige	description powder (fine), ~200 U/g
specific activity ~50 U/mg	specific activity ≥20,000 U/g	specific activity ≥100,000 U/g	specific activity ~200 U/g
form powder	form lyophilized	form solution	form powder (fine)
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
color white	color -	color beige, white	color -
Quality Level 100	Quality Level 200	Quality Level 200	Quality Level 100
quality lyophilized	quality -	quality -	quality -

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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