71077
X-Gal Solution - Novagen
Convenience for blue/white screening
Synonym(s):
X-Gal solution
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|
About This Item
NACRES:
NA.54
UNSPSC Code:
12352200
Pricing and availability is not currently available.
Quality Segment
form
solution
manufacturer/tradename
Novagen®
storage condition
OK to freeze
technique(s)
tissue culture: suitable
shipped in
wet ice
storage temp.
−20°C
General description
X-Gal, or 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, is an organic compound commonly used in molecular biology as a chromogenic substrate for the enzyme β-galactosidase, which is encoded by the LacZ gene. When β-galactosidase hydrolyzes X-Gal, it forms galactose and 5,5′-dibromo-4,4′-dichloro-indigo, resulting in the formation of intense blue color colonies. This process is widely used in LacZ activity assays and staining techniques, such as X-gal staining or LacZ staining to distinguish between recombinants (white) and nonrecombinants (blue). X-Gal staining is frequently used in the fields of molecular biology, genetics, and developmental biology to study gene expression and cell line tracing. X-Gal Solution is provided as a convenient 40 mg/ml concentrate in DMSO, ready for dilution into the culture medium or appropriate buffers.
Application
X-Gal Solution - Novagen has been used at 1:40 dilution to detect the expression of β-galactosidase and mutant fusion protein generated by the ApcΔ242 mutation in the tissue sample.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
1 of 1
This Item | |||
|---|---|---|---|
| description Convenience for blue/white screening | description Convenient, ready-to-use solution | description reagent for selection of recombinant bacterial clones | description reagent for selection of recombinant bacterial clones |
| technique(s) tissue culture: suitable | technique(s) - | technique(s) nucleic acid detection: suitable | technique(s) - |
| form solution | form solution | form powder | form powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. room temp |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in - |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| storage condition OK to freeze | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition - | storage condition - |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
188.6 °F
flash_point_c
87 °C
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.