These 5 mg pNPP tablets can be dissolved in water or aqueous buffer solutions (e.g., 50 mM Tris buffer, pH 7.0). For a typical ELISA assay, a working concentration range of 1 to 10 mg/ml is generally used, with the stock concentration being 5 to 10 times its working concentration. Preparing a 0.5 ml solution with one tablet results in a concentration of 10 mg/ml.
487666
p-Nitrophenyl Phosphate Tablets
Excellent substrate for alkaline and acid phosphatase-based ELISA assays.
Synonym(s):
p-Nitrophenyl Phosphate Tablets, pNPP
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About This Item
Form:
(24 Tablets)
Storage condition:
OK to freeze
Pricing and availability is not currently available.
Quality Segment
form
(24 Tablets)
packaging
pkg of 24 tablets
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
shipped in
ambient
storage temp.
−20°C
General description
Each tablet contains 5 mg pNPP.
Excellent substrate for alkaline and acid phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm. Both kinetic and endpoint determinations can be performed.
Excellent substrate for alkaline and phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm. Both kinetic and endpoint determinations can be performed. Each tablet contains 5 mg pNPP.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
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This Item | |||
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| description Excellent substrate for alkaline and acid phosphatase-based ELISA assays. | description Excellent substrate for alkaline phosphatase-based ELISA assays. | description p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate, CAS 4264-83-9, is an excellent substrate for alkaline phosphatase-based ELISA assays. Produces yellow soluble end product (405-410 nM). | description Substrate for the colorimetric assay of β-D-xylosidase. Also shown to disrupt proteoglycan biosynthesis in vivo. |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| storage condition OK to freeze | storage condition OK to freeze | storage condition OK to freeze, protect from light | storage condition OK to freeze |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| packaging pkg of 24 tablets | packaging - | packaging - | packaging - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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