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1.08178

Trifluoroacetic acid

for protein sequence analysis

Synonym(s):

TFA

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About This Item

Linear Formula:
CF3COOH
CAS Number:
Molecular Weight:
114.02
UNSPSC Code:
12352106
NACRES:
NA.21
EC Index Number:
200-929-3
MDL number:
Beilstein/REAXYS Number:
742035
Assay:
≥99.7% (acidimetric)
Form:
liquid
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vapor density

3.9 (vs air)

Quality Segment

vapor pressure

97.5 mmHg ( 20 °C)

assay

≥99.7% (acidimetric)

form

liquid

impurities

≤0.02% Water (Karl Fischer)

refractive index

n20/D 1.3 (lit.)

pH

1 (10 g/L in H2O)

bp

72.4 °C (lit.)

mp

−15.4 °C (lit.)

solubility

soluble 10 g/mL

density

1.489 g/mL at 20 °C (lit.)

storage temp.

2-30°C

SMILES string

OC(C(F)(F)F)=O

InChI

1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)

InChI key

DTQVDTLACAAQTR-UHFFFAOYSA-N

Application

Trifluoroacetic acid (TFA) can be used as an ion-pairing agent in a reverse-phase HPLC system to separate peptides because it enhances peak resolution. It also has low absorption detection wavelengths and is relatively volatile and can be easily removed. For the majority of peptide separations, TFA has been used at concentrations ranging from 0.05 to 0.1%(6.5-13 mM).[1]

Analysis Note

Assay (acidimetric): ≥ 99.7 %
Identity (IR-spectrum): passes test
Density (d 20 °C/20 °C): about 1.53
Boiling point: about 72 °C
Water (according to Karl Fischer): ≤ 0.02 %

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This Item
2995371.0826291707
assay

≥99.7% (acidimetric)

assay

≥99%

assay

≥99.8% (acidimetric)

assay

≥99.0% (GC)

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

2-30°C

storage temp.

-

storage temp.

2-30°C

storage temp.

-

pH

1 (10 g/L in H2O)

pH

1 (20 °C, 10 g/L)

pH

1 (10 g/L in H2O)

pH

1 (20 °C, 1 g/L)

solubility

soluble 10 g/mL

solubility

water: soluble 10.000 g/L

solubility

soluble 10 g/mL

solubility

water: soluble


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CorrosionExclamation mark

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Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 2



Certificates of Analysis (COA)

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Y Chen et al.
Journal of chromatography. A, 1043(1), 9-18 (2004-08-20)
Trifluoroacetic acid (TFA) remains the dominant mobile phase additive for reversed-phase high-performance liquid chromatography (RP-HPLC) of peptides after more than two decades since its introduction to this field. Generally, TFA has been employed in a concentration range of 0.05-0.1% (6.5-13



Global Trade Item Number

SKUGTIN
108178005004022536101309
108178000004022536101293

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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