G005644

HPLC Analysis of Nucleosides on Ascentis^® Express OH5 (HILIC Mode)

application for HPLC

Propriétés

• Technique(s): HPLC: suitable
• Paramètres du test: column: Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53757-U); mobile phase: [A] 5 mM ammonium acetate, pH 5.0 with acetic acid in 95:5, acetonitrile:water; [B] 5 mM ammonium acetate, pH 5.0 with acetic acid in 80:20, acetonitrile:water; gradient: 0% B held for 1 min; to 100% B in 10 min; held at 100% B for 1 min; flow rate: 0.3 mL/min; column temp.: 25 °C; detector: UV, 250 nm; injection: 2 μL; sample: 10 - 100 μg/mL in 95:5, acetonitrile:water (pH of buffer stock (in water) was adjusted before further dilution with water and/or acentonitrile)
• Adéquation: application for HPLC
• Application(s): life science and biopharma

Description

Remarque sur l'analyse

Nucleosides are involved in many aspects of metabolism. Analogs of nucleosides have medical application in antivirals or anticancer treatments. This application demonstrates the utility of hydrophilic interaction chromatography (HILIC) for the retention and resolution of nucleosides and analogs thereof. Elution is not solely explained by logD but by other ionic and solvophobic effects.

Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

5-Methyluridine
DMT-2′O-TC-rU Phosphoramidite
2-Thiocytidine dihydrate
2′-O-Methylcytidine
Inosine
5-Methylcytidine
Cytidine
DMT-2′O-TC-rG(ib) Phosphoramidite
1-Methyladenosine
7-Methylguanosine
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