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581325-U

Supelco

Ascentis® C18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Synonyme(s) :

Ascentis RP18 HPLC Column

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About This Item

Code UNSPSC :
41115700
eCl@ss :
32110501
Nomenclature NACRES :
SB.52

product name

Colonne Ascentis® C18 pour HPLC, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

Matériaux

stainless steel column

Niveau de qualité

100

Agence

suitable for USP L1 (Similar to Phenomenex Luna C18)

Gamme de produits

Ascentis®

Caractéristiques

endcapped

Fabricant/nom de marque

Ascentis®

Conditionnement

1 ea of

Ampleur du marquage

25% Carbon loading

Paramètres

≤70 °C temp. range
400 bar pressure (5801 psi)

Technique(s)

HPLC: suitable
LC/MS: suitable

L × D.I.

25 cm × 4.6 mm

Superficie

450 m2/g

Couverture de surface

3.7 μmol/m2

Impuretés

<5 ppm metals

Matrice

fully porous particle
silica gel high purity, spherical

Groupe de la matrice active

C18 (octadecyl) phase

Taille des particules

5 μm

Dimension de pores

100 Å

operating pH range

2-8

Application(s)

food and beverages

Technique de séparation

reversed phase

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Description générale

Ascentis C18 est une colonne de première intention pour HPLC, extrêmement stable et fiable, qui donne des pics de forme symétrique et une excellente rétention, y compris pour des composés difficiles.

Application


  • Identification of recombinant human insulin and biosynthetic insulin analogues by multiplexed targeted unlabeled mass spectrometry of proteotypic tryptic peptides.: This study utilized the Ascentis® C18 HPLC column for the separation of proteotypic tryptic peptides in the identification of insulin analogues, highlighting its application in targeted mass spectrometry for proteomic analysis (Qasem RJ et al., 2019).

  • Development and validation of a novel stability-indicating HPLC method for the simultaneous assay of betamethasone-17-valerate, fusidic acid, potassium sorbate, methylparaben and propylparaben in a topical cream preparation.: This research developed and validated an HPLC method using the Ascentis® C18 HPLC column for the simultaneous assay of multiple compounds, demonstrating its robustness in stability testing of pharmaceutical formulations (Byrne J et al., 2014).

  • HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts.: The study employed the Ascentis® C18 HPLC column for metabolite profiling of propolis extracts using HPLC-DAD and HPLC-ESI-MS/MS, showcasing its effectiveness in complex natural product analysis (Pellati F et al., 2011).

  • Practical comparison of 2.7 microm fused-core silica particles and porous sub-2 microm particles for fast separations in pharmaceutical process development.: This article compared different HPLC column technologies, including the Ascentis® C18, highlighting its performance in fast separations critical for pharmaceutical process development (Abrahim A et al., 2010).

Caractéristiques et avantages

  • Excellente rétention
  • Forme symétrique du pic
  • Forte reproducibilité
  • Compatibilité totale LC-MS

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Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
292677-06
292677-05
292677-04
292677-03
292677-02

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Michael Kühl et al.
eLife, 9 (2020-01-22)
Far-red absorbing chlorophylls are constitutively present as chlorophyll (Chl) d in the cyanobacterium Acaryochloris marina, or dynamically expressed by synthesis of Chl f, red-shifted phycobiliproteins and minor amounts of Chl d via far-red light photoacclimation in a range of cyanobacteria
Federica Pellati et al.
Journal of chromatography. A, 1242, 43-58 (2012-05-09)
In this study, a detailed phytochemical characterization of Echinacea pallida (Nutt.) Nutt. root extracts and dietary supplements was carried out for the first time by developing advanced chromatographic techniques, based on HPLC with diode array (DAD) and electrospray ionization-mass spectrometry
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 55(5), 934-948 (2011-04-19)
In this study, the composition of polyphenols (phenolic acids and flavonoids) in propolis extracts was investigated by HPLC-DAD and HPLC-ESI-MS/MS by comparing the performance of ion trap and triple quadrupole mass analyzers. The analyses were carried out on an Ascentis
Dipanjan Goswami et al.
Biomedical chromatography : BMC, 23(11), 1227-1241 (2009-07-14)
A LC-MS/MS method for plasma topiramate analysis is delineated involving least number of healthy volunteers. Topiramate and amlodipine internal standard (IS) were extracted by simple centrifuge-coupled solid-phase extraction and reverse-phase chromatographic separation was performed on an Ascentis C(18) column. Turbo-spray
Meiyun Shi et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 951-952, 129-134 (2014-02-22)
Aralia mandshrica is a well-known traditional Chinese medicine from Northeast China commonly used to treat digestive, circulatory and immune system disorders. Calenduloside E is one of its bioactive components currently under evaluation as a pure drug. In this study, a
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Protocoles

US EPA Method 632: HPLC Analysis of Pesticides on Ascentis® C18

-methylcarbamate 10 μg/mL; Diuron; Propham; Siduron; Methiocarb, analytical standard; Linuron 10 μg/mL; Swep 10 μg/mL; Chlorpropham 10 μg/mL; Barban; Neburon

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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