V8379

Human Vitronectin

from human plasma, powder, suitable for cell culture

• Synonyme(s) : Serum spreading factor
• Numéro CAS: 83380-82-9
• Numéro MDL: MFCD00132575
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.75

Propriétés

• product name: Vitronectin from human plasma, lyophilized powder, BioReagent, suitable for cell culture
• Source biologique: human plasma
• Niveau de qualité: 300
• Gamme de produits: BioReagent
• Forme: lyophilized powder
• Poids mol.: A subunit 75 kDa; B subunit 65 kDa
• Conditionnement: pkg of 50 μg
• Technique(s): cell culture | mammalian: suitable
• Couverture de surface: 0.1 μg/cm²
• Solubilité: water: soluble 0.050 mg/mL, clear, colorless
• Numéro d'accès UniProt: P04004
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Informations sur le gène: human ... VTN(7448)

Description

Application

Vitronectin can be used with cells with integrin receptors that bind vitronectin, including platelets, endothelial cells, melanoma cells, and osteosarcoma. It is recommended as a cell culture substratum at 0.1 μg/cm². Optimal conditions for attachment depend on cell line and application.

Actions biochimiques/physiologiques

Vitronectin binds to glycosaminoglycans, is incorporated as an inhibitor to the membrane cytolytic attack complex of the complement system, has interaction with thrombin and antithrombin III during coagulation, and may play a role in the coagulation pathway.

Composants

Vitronectin is a glycoprotein present in plasma and tissues, and is one of the major cell adhesion proteins, along with fibronectin, in plasma. The N-terminal residues of vitronectin are identical to somatomedin B and are followed by an R-G-D sequence that interacts with a specific cell-surface receptor. The central domain of vitronectin is enriched with hydrophobic residues. Closer to the C-terminus, it has an arginine rich region that allows for heparin binding activity.

Attention

The lyophilized product should be stored desiccated at 2-8°C. Under these conditions it is stable for at least 2 years. Aliquots should be stored at -20°C or -70°C, but repeated freezing and thawing should be avoided.

Notes préparatoires

This product is supplied as a cell culture tested lyophilized powder from buffered saline. It is recommended to use polypropylene vessels during handling, due to the strong affinity of vitronectin for hydrophilic surfaces. Reconstitution of this product can be done with 1 mL of water per vial. The stock solution should then be filter-sterilized using a 0.2μm membrane and aliquoted and frozen for long term storage.

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du ministère français de la recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable