SRP3189

APRIL from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonyme(s) : CD256, TALL-2, ZTNF2, A Proliferating-inducing Ligand, TNFSF13, TRDL-1a
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: mouse
• Produit recombinant: expressed in E. coli
• Pureté: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Forme: lyophilized
• Poids mol.: 21.9 kDa
• Conditionnement: pkg of 20 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: <0.1 EU/μg endotoxin, tested
• Couleur: white to off-white
• Numéro d'accès Protein ID: AAH69900
• Numéro d'accès UniProt: Q9D777
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: mouse ... TNFSF13(69583)

Description

Description générale

Research area: Immunology and Cytokines

APRIL (a proliferation-inducing ligand) was identified in 1998 through database mining and is a member of the TNF (tumor necrosis factor) family. APRIL gene is localized to human chromosome 17p13, contains six exons, and is alternatively transcribed to 1.8, 2.1, and 2.4 kb mRNA transcripts. APRIL protein is composed of a cytosolic domain of 28 amino acids, and a transmembrane region. It also contains an exoplasmic region, made up of a stalk and a TNF domain. It shares the highest similarity to BLyS (B lymphocyte stimulator) protein, ~30% sequence similarity in the TNF domain. It is synthesized as a type II transmembrane protein and is proteolytically processed as a mature protein, which is a soluble and non-covalent trimer. Recombinant murine APRIL is a soluble 21.9 kDa protein, consisting of 192 amino acid residues.

Actions biochimiques/physiologiques

A proliferation-inducing ligand (APRIL) and its ligand interaction activate several signaling pathways, including nuclear factor-kappa B (NF-κB), p38, c-Jun N-terminal kinase mitogen-activated protein kinase (JNK MAPK), and Protein Kinase B/Forkhead Box O (AKT/FOXO), which are necessary for promoting the survival and differentiation of B cells and plasma cells. Additionally, APRIL signaling aids in the regulation of cytokine production, the generation of humoral immunity, decreases T cell-dependent antibody responses, and facilitates IgA class switching. These functions highlight the crucial role of APRIL in regulating immune responses. Furthermore, APRIL is also known to induce immune cell apoptosis leading to immunosuppression, and tumor development.

Séquence

MRREVSRLQR SGGPSQKQGE RPWQSLWEQS PDVLEAWKDG AKSRRRRAVL TQKHKKKHSV LHLVPVNITS KDSDVTEVMW QPVLRRGRGL EAQGDIVRVW DTGIYLLYSQ VLFHDVTFTM GQVVSREGQG RRETLFRCIR SMPSDPDRAY NSCYSAGVFH LHQGDIITVK IPRANAKLSL SPHGTFLGFV KL

Forme physique

Lyophilized from 10 mM Sodium Acetate, pH 5.0 + 100 mM Arginine.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable