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Key Documents

SRP2145

Sigma-Aldrich

LRH-1, GST tagged human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonyme(s) :

B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.26

Source biologique

human

Produit recombinant

expressed in E. coli

Pureté

≥80% (SDS-PAGE)

Forme

frozen liquid

Poids mol.

~83 kDa

Conditionnement

pkg of 10 μg

Concentration

500 μg/mL

Couleur

clear colorless

Numéro d'accès NCBI

NM_003822

Numéro d'accès UniProt

O00482

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... NR5A(2494)

Actions biochimiques/physiologiques

LRH-1 (NR5A2) is expressed specifically in pancreas and liver, playing important roles in the regulation of several liver-specific genes. LRH-1 is a mammalian homologue of Drosophila fushi tarazu factor (dFTZ-F1) and structurally belongs to the orphan nuclear receptor superfamily. LRH-1 can recognize the DNA sequence 5′-AAGGTCA-3′, the canonical recognition motif for steroidogenic factor 1 (SF-1). Enhancer II (ENII) is one of the critical cis-elements in the Hepatitis B Virus (HBV) genome for the hepatic viral gene transcription and DNA replication. The liver-specific activity of ENII is regulated by multiple liver-enriched transcription factors, including LRH-1/hB1F. The role LRH-1 and dosage-sensitive sex reversal, adrenal hypoplasia congenital critical region on the X chromosome, gene 1 (DAX-1) in the regulation of StAR gene expression in human granulosa cell tumor cells has been investigated. LRH-1, is expressed in granulosa cells and has been shown to synergize with the cAMP signaling system to regulate the gonadal type II aromatase promoter in transient transfection assays. Observations support a direct role for LRH-1 in the induction of the progesterone but not the estrogen biosynthetic pathway during granulosa cell differentiation. The human nuclear receptor liver receptor homolog 1 (hLRH-1) plays an important role in the development of breast carcinomas. results indicate that hLRH-1′s control of gene expression is mediated by phospholipid binding, and establish hLRH-1 as a novel target for compounds designed to slow breast cancer development.

Forme physique

Clear and colorless frozen liquid solution

Notes préparatoires

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Wang, Z.N., et al.
Journal of Molecular Endocrinology, 2, 255-258 (2001)
C K Zhang et al.
Gene, 273(2), 239-249 (2001-10-12)
The human homologue of the Drosophila melanogaster orphan nuclear receptor fushi tarazu factor 1 (Ftz-F1), NR5A2 (hB1F), was initially identified as a regulatory factor that binds and activates enhancer II of hepatitis B virus. NR5A2 (hB1F) is expressed specifically in
Yan Ning Cai et al.
Cell research, 13(6), 451-458 (2004-01-20)
Enhancer II (ENII) is one of the critical cis-elements in the Hepatitis B Virus (HBV) genome for the hepatic viral gene transcription and DNA replication. The liver-specific activity of ENII is regulated by multiple liver-enriched transcription factors, including LRH-1/hB1F, HNF1

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