SRP0721

AKT1 (E17K), Active

PRECISIO^® Kinase, recombinant

• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Produit recombinant: expressed in baculovirus infected Sf9 cells
• Pureté: 70-95% (SDS-PAGE)
• Forme: frozen liquid
• Activité spécifique: 50-75 nmol/min·mg (radiometric)
• Poids mol.: ~85 kDa
• Concentration: 0.1 μg/μL
• Numéro d'accès: NM_005163
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... AKT1(207)

Description

Application

Useful for kinase assay.

Actions biochimiques/physiologiques

Recombinant full-length human AKT1 (E17K) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. AKT1/PKBalpha is a serine/threonine kinase that belongs to the AKT family. AKT1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility ⁽¹⁾. AKT1 is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias ⁽²⁾.

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Stockage et stabilité

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Informations légales

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable