SRP0277

PDE10A1 Active human

recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)

• Synonyme(s) : HSPDE10A, Phosphodiesterase 10A1, cAMP and cAMP-inhibited cGMP 3′,5′-cyclic phosphodiesterase 10A
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in baculovirus infected insect cells
• Pureté: ≥60% (SDS-PAGE)
• Forme: aqueous solution
• Activité spécifique: ≥2851 pmol/min-μg
• Poids mol.: 116 kDa
• Conditionnement: pkg of 10 μg
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: NM_006661
• Numéro d'accès UniProt: Q9Y233
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... PDE10A(10846)

Description

Description générale

Human PDE10A1 (GenBank Accession No. NM_006661), full length with N-terminal GST tag, MW = 116 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Définition de l'unité

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable