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SRP0158

Sigma-Aldrich

Histone H3 (2-58) human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonyme(s) :

HIST1H3E, Histone H3.1

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

human

Produit recombinant

expressed in E. coli

Essai

≥70% (SDS-PAGE)

Forme

aqueous solution

Poids mol.

32 kDa

Conditionnement

pkg of 500 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Concentration

>0.02 mg/mL

Numéro d'accès NCBI

NM_003532

Numéro d'accès UniProt

P68431

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... HIST1H3E(8353)

Description générale

The mammalian chromatin is present in nucleosomes, made of histone octamers (H2A,H2B,H3,H4)2. Mammals contain three main classes of histone H3 variants: the replicative histones (H3.1 and H3.2), the replacement histone (H3.3) and the centromeric histone (Cenp-A).[1][2][3]
Human Histone 3 (GenBank Accession No. NM_003532), (amino acid 2-58) with N-terminal GST tag, MW = 32kDa, expressed in an Escherichia coli expression system.

Application

Suitable substrate for histone methyltransferases

Actions biochimiques/physiologiques

Histone proteins are basic building blocks of chromatin.[4] H3.1 (also referred to as HIST1H3E) is mainly expressed in the S phase and is termed as replication-dependent histone.[5] Selective methylation of H3.1 controls replication in heterochromatin area.[6]

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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Certificats d'analyse (COA)

Lot/Batch Number
52013090209
5201390209

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Consulter la Bibliothèque de documents

Selective methylation of histone H3 variant H3.1 regulates heterochromatin replication.
Jacob Y, et al.
Science, 343, 1249-1249 (2014)
Topography of the histone octamer surface: repeating structural motifs utilized in the docking of nucleosomal DNA.
Arents G and Moudrianakis EN
Proceedings of the National Academy of Sciences of the USA, 90, 10489-10489 (1993)
Genome-wide analysis of histone H3 lysine 4 trimethylation in peripheral blood mononuclear cells of minimal change nephrotic syndrome patients.
Zhang L, et al.
American Journal of Nephrology, 30, 505-505 (2009)
Eric I Campos et al.
Nature structural & molecular biology, 17(11), 1343-1351 (2010-10-19)
The mechanism by which newly synthesized histones are imported into the nucleus and deposited onto replicating chromatin alongside segregating nucleosomal counterparts is poorly understood, yet this program is expected to bear on the putative epigenetic nature of histone post-translational modifications.
Alejandra Loyola et al.
Molecular cell, 24(2), 309-316 (2006-10-21)
Histone posttranslational modifications (PTMs) and sequence variants regulate genome function. Although accumulating evidence links particular PTM patterns with specific genomic loci, our knowledge concerning where and when these PTMs are imposed remains limited. Here, we find that lysine methylation is
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Questions

1–2 of 2 Questions  

  1. What is the amino acid sequence of Histone H3 (2-58) human, product SRP0158?

    1 answer

    1. The sequence for product SRP0158, Histone H3 (2-58) human, is as follows:ARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKS

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  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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