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SH3011

Sigma-Aldrich

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock

Epigenetic Regulators

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About This Item

Code UNSPSC :
41106609
Nomenclature NACRES :
NA.51

Gamme de produits

MISSION®

Température de stockage

−70°C

Informations sur le gène

human ... CA1(759) , DNMT1(1786) , DNMT3A(1788) , DNMT3B(1789) , DNMT3L(29947) , MGMT(4255) , N6AMT1(29104) , N6AMT2(221143) , SPEM1(374768) , TRDMT1(1787)

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Description générale

The clones are sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) provided as 50 μl bacterial glycerol stocks (Terrific Broth, carbenicillin at 100 μg/ml and 15% glycerol). The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

Autres remarques

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Number of Genes: 11, Number of Clones: 57
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.

Informations légales

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Epigenetics in cancer.
Manel Esteller
The New England journal of medicine, 358(11), 1148-1159 (2008-03-14)
Kirsten Grønbaek et al.
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 115(10), 1039-1059 (2007-11-29)
A cancer develops when a cell acquires specific growth advantages through the stepwise accumulation of heritable changes in gene function. Basically, this process is directed by changes in two different classes of genes: Tumor suppressor genes that inhibit cell growth

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