This antibody targets an epitope located in amino acid residues 4-10 at the N-terminus of the Opsin (rhodopsin) protein. As the retinal group is covalently linked to the opsin protein component on its lysine residues and the epitope region (AA 4-10) does not contain any lysine, it is unlikely that a retinal group is involved in the reactivity of this antibody. Therefore, the reactivity of this antibody would only involve the opsin component of the observed rhodopsin protein (UniProt#: P08100) upon labeling.
SAB4200762
Anti-Opsin antibody, Mouse monoclonal
clone RET-P1, purified from hybridoma cell culture
Synonyme(s) :
Anti-Opsin 2, Anti-Rhodopsin
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified from hybridoma cell culture
Type de produit anticorps
primary antibodies
Clone
RET-P1, monoclonal
Forme
buffered aqueous solution
Poids mol.
~39 kDa
Espèces réactives
avian, bovine, fish, mouse, rat, human, amphibian, turtle, rabbit
Concentration
~1 mg/mL
Technique(s)
ELISA: suitable
immunoblotting: 2-4 μg/mL using human retinoblastoma Y79 cell line extract.
immunofluorescence: suitable
immunohistochemistry: 5-10 μg/mL using rat eye frozen sections.
radioimmunoassay: suitable
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
rat ... Rho(24717)
Catégories apparentées
Description générale
Anti-Opsin antibody, Mouse monoclonal (rhodopsin) (mouse IgG1 isotype) is derived from the RET-P1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/C mice immunized with rat retinal membranes. Opsin is also called as rhodopsin protein, members of the G-protein coupled receptor (GPCR) superfamily. Opsin comprises >95% of the rod outer segments (ROS) intrinsic membrane and consists of a protein moiety - an opsin - and a non-protein moiety - the chromophore retinal.
Application
Anti-Opsin antibody has been used in
- immunoblotting
- immunofluorescence
- immunohistochemistry
- enzyme linked immunosorbent assay (ELISA)
- radioimmuno assay (RIA)
Actions biochimiques/physiologiques
Opsin is an essential molecules for mediating the ability of animals to detect and use light for diverse biological functions. Mutations in the opsin (rhodopsin) gene are linked to retinitis pigmentosa (RP), a disease characterized by retinal degeneration resulting in reduced peripheral vision and night blindness.
Autres remarques
rat retinal membranes
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Certificats d'analyse (COA)
Lot/Batch Number
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Retinitis pigmentosa
Hamel C.
Orphanet Journal of Rare Diseases, 1(1), 40-40 (2006)
The opsins
Terakita A.
Genome Biology, 6(3), 213-213 (2005)
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I am confused by the wording in the description of the product on your website: "Anti-Opsin antibody, Mouse monoclonal (rhodopsin) (mouse IgG1 isotype) is derived from the RET-P1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/C mice immunized with rat retinal membranes. Opsin is also called as rhodopsin protein, members of the G-protein coupled receptor (GPCR) superfamily. Opsin comprises >95% of the rod outer segments (ROS) intrinsic membrane and consists of a protein moiety - an opsin - and a non-protein moiety - the chromophore retinal." Does this imply that this anti-opsin will recognize Rhodopsin as well, i.e. when we observe labeling for opsin using this antibody we could be observing Rhodopsin?
1 answer-
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