SAB4200731

Anti-Pan-Cadherin antibody, Mouse monoclonal

clone CH-19, purified from hybridoma cell culture

• Synonyme(s) : Anti-Pan Cadherin
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: CH-19, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: ~135 kDa
• Espèces réactives: guinea pig, canine, frog, bovine, human, cat, monkey, rabbit, mouse, ferret, snake, hamster, chicken, Psammomys (sand rat), rat
• Concentration: ~1.0 mg/mL
• Technique(s): immunoblotting: 0.125-0.25 μg/mL using HeLa cell extract; immunofluorescence: 5-10 μg/mL using HeLa cells; immunohistochemistry: suitable
• Isotype: IgG1
• Numéro d'accès UniProt: P12830; P19022; P22223
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CDH1(999)

Description

Description générale

Anti-Pan-Cadherin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the CH-19 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with the synthetic peptide.

N-cadherin, also known as cadherin-2 (CDH2) or neural cadherin (NCAD) is mapped to human chromosome 18q12.1. N-cadherin has ectodomains comprising of 110 amino acidsand is called the extracellular cadherin (EC) domain. EC domains are critical for the adhesion function of N-cadherin.

Spécificité

Monoclonal Anti-Pan-Cadherin recognizes Cadherin from human, mouse, monkey, bovine, chicken, rat, ferret, canine, rabbit, pig, guinea pig, hamster, cat, frog, snake and psammomys origin. Monoclonal Anti Pan-Cadherin is reactive against a highly conserved sequence at the cytoplasmic C-terminal of N-Cadherin protein.

Immunogène

synthetic peptide corresponding to the C-terminus of chicken N-Cadherin with an extra N-terminal lysine residue coupled with KLH.

Application

Anti-Pan-Cadherin antibody, Mouse monoclonal may be used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

Actions biochimiques/physiologiques

N-cadherin plays a key role in the neurulation, which involves transition of neural plate into neural tube. It is present in the synaptic membrane and dimerises upon synaptic induction and mediates synaptic activity. N-cadherin mediates cell−cell adhesion in retina but favors tumor progression in retinoblastoma. It is upregulated in carcinomas and is critical for cell invasion. Irregular distribution of N-cadherin is implicated in dilated cardiomyopathy. Mutations in the CHD2 gene may probably contribute to obsessive-compulsive disorder (OCD) and Tourette disorder (TD).

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable