SAB4200690

Anti-EpCAM antibody, Mouse monoclonal

clone Ber-EP4, hybridoma cell culture supernatant

• Synonyme(s) : Anti-CD326 antigen, Anti-EGP, Anti-Epithelial cell surface antigen, Anti-Epithelial glycoprotein, Anti-KS 1/4 antigen, Anti-KSA, Anti-Major gastrointestinal tumor-associated protein GA733-2, Anti-Tumor-associated calcium signal transducer 1
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: culture supernatant
• Type de produit anticorps: primary antibodies
• Clone: Ber-EP4, monoclonal
• Forme: buffered aqueous solution
• Espèces réactives: human
• Technique(s): immunofluorescence: 1:500-1:1,000 using human breast adenocarcinoma MCF-7 cell line. ; immunohistochemistry: 1:250-1:500 using heat-retrieved formalin-fixed, paraffin-embedded human colon carcinoma sections.; immunoprecipitation (IP): suitable
• Isotype: IgG1
• Numéro d'accès UniProt: P16422
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... EPCAM(4072)

Description

Description générale

Anti-EpCAM antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma Ber-EP4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human breast carcinoma cell line MCF-7. EpCAM (Epithelial cell adhesion molecule) is a highly conserved type I transmembrane glycoprotein. EpCAM is present on most epithelia tissues of the adult body and in undifferentiated rather than differentiated embryonic stem cells.

Immunogène

human breast carcinoma cell line MCF-7

Application

Anti-EpCAM antibody has been used in:

• immunofluorescence
• immunohistochemistry
• immunoprecipitation

Actions biochimiques/physiologiques

EpCAM (Epithelial cell adhesion molecule) regulates cell-cell contact adhesion strength and tissue plasticity and epithelial cell proliferation and differentiation. Mutations in EpCAM are associated with several intestinal abnormalities such as Lynch syndrome, and congenital tufting enteropathy (CTE). Anti-EpCAM has been proved valuable for the distinction of undifferentiated primary or metastatic tumors from non-epithelial tumors, bile duct cells from hepatocytes in certain liver diseases, and between epithelial and normal reactive or neoplastic mesothelial cells from carcinoma cells.

Forme physique

The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains bovine serum albumin and a human-derived protein.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable