SAB4200631

Monoclonal Anti-CYBA antibody produced in mouse

clone CB-64, purified from hybridoma cell culture

• Synonyme(s) : Cytochrome b558 subunit alpha, Neutrophil cytochrome b 22 kDa polypeptide, Superoxide-generating NADPH oxidase light chain subunit, cytochrome b-245 alpha polypeptide, p22-PHOX
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: CB-64, monoclonal
• Forme: buffered aqueous solution
• Espèces réactives: bovine, mouse, rat, monkey, human, canine
• Concentration: ~1 mg/mL
• Technique(s): flow cytometry: 5-10 μg/test using HeLa cells.; immunoblotting: 0.5-1 μg/mL using whole extracts of HeLa cells.; immunofluorescence: 5-10 μg/mL using HeLa cells.
• Isotype: IgM
• Numéro d'accès UniProt: P13498
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CYBA(1535) ; mouse ... Cyba(13057) ; rat ... Cyba(79129)

Description

Description générale

Monoclonal Anti-CYBA (mouse IgM isotype) is derived from the hybridoma CB-64 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the internal region of human CYBA, conjugated to keyhole limpet hemocyanin (KLH). The small subunit, p22phox subunit, also known as cytochrome b245, alpha polypeptide/light chain (CYBA) is membrane bound,which is expressed in phagocytic, endothelial and vascular smooth muscle cells. This gene is located on human chromosome16q24

Immunogène

Synthetic peptide corresponding to a sequence at the internal region of human CYBA .

Application

Monoclonal Anti-CYBA antibody produced in mouse has been used in:

• immunoblotting
• flow cytometry
• immunofluorescence

Actions biochimiques/physiologiques

In prostate cancer cells downregulation of cytochrome b245, alpha polypeptide/light chain (CYBA) was reported to inhibited cell proliferation and colony formation, through AKT and ERK1/2 signaling pathways, indicating CYBA has a role in tumor angiogenesis and tumor growth. Mutations in this gene causes an autosomal recessive forms of chronic granulomatous disease (AR220CGD).

Forme physique

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable