SAB4200544

Anti-Synaptophysin antibody, Mouse monoclonal

clone SVP-38, purified from hybridoma cell culture

• Synonyme(s) : Monoclonal Anti-MRXSYP, Monoclonal Anti-SYP, Monoclonal Anti-Synaptophysin1, Monoclonal Anti-Synaptoporin, Monoclonal Anti-Syp1, Monoclonal Anti-p38
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Conjugué: unconjugated
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: SVP-38, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 38 kDa
• Espèces réactives: rat, human
• Concentration: ~1.0 mg/mL
• Technique(s): immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded rat cerebellum.; indirect immunofluorescence: 10-20 μg/mL using B35 cells.; western blot: 1-2 μg/mL using newborn rat brain extracts.
• Isotype: IgG1
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SYP(6855)

Description

Description générale

Anti-Synaptophysin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma SVP-38 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synaptosome preparation from rat retina. Synaptophysin is a glycoprotein containing four transmembrane domains with both N and C termini on the cytoplasm face.

Synaptophysin (SYP) is encoded by the gene mapped to human chromosome Xp11.23-p11.22. The encoded protein has a molecular mass of 38kDa and is exclusively localized to the membrane of presynaptic vesicles.

Spécificité

Anti-Synaptophysin antibody, Mouse monoclonal recognizes rat, human, guinea-pig, and pig synaptophysin

Immunogène

synaptosome preparation from rat retina. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor.

Application

Anti-Synaptophysin antibody, Mouse monoclonal has been used in following immunochemical techniques:

• Immunoblotting
• Immunohistochemistry
• Immunofluorescence

Actions biochimiques/physiologiques

Synaptophysin (SYP) facilitates neurotransmitter release and synaptic plasticity. It is also implicated in synaptic vesicle formation and exocytosis. Mutation in the gene increases the risk of susceptibility to schizophrenia. It is widely used as a potential marker to study the circulation of synapses in the brain and to discover the basic features of the life cycle of synaptic vesicles (SVs).

Synaptophysin has also been implicated in the recycling of synaptic vesicles by associating with dynamin I, a GTPase required for endocytosis.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable