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Key Documents

SAB4200454

Sigma-Aldrich

Anti-FUS antibody produced in rabbit

enhanced validation

~1.0 mg/mL, affinity isolated antibody

Synonyme(s) :

Anti-ALS6, Anti-CHOP, Anti-FUS-CHOP, Anti-FUS1, Anti-TLS, Anti-TLS/CHOP, Anti-hnRNP-P2

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~68 kDa

Espèces réactives

rat, human

Validation améliorée

independent
Learn more about Antibody Enhanced Validation

Concentration

~1.0 mg/mL

Technique(s)

immunohistochemistry: 5-10 μg/mL using formalin-fixed paraffin embedded rat colon.
indirect immunofluorescence: 2.5-5 μg/mL using HeLa cells.
western blot: 1.5-3.0 μg/mL using using lysates of Jurkat cells.

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... FUS(2521)
rat ... Fus(317385)

Description générale

Fused in sarcoma (FUS) is a component of heterogeneous nuclear ribonucleoprotein (hnRNP) complex is a DNA/ RNA binding protein. FUS gene is mapped to human chromosome 16p11.2 and is located predominantly in the nucleus.

Spécificité

Anti-FUS specifically recognizes human and rat FUS.

Immunogène

synthetic peptide corresponding to the N-terminal region of human FUS isoform 1, conjugated to KLH. The corresponding sequence is identical in human FUS isoforms 2 and 3, and highly conserved (single amino acid insertion) in mouse and rat FUS.

Application

Anti-FUS antibody produced in rabbit has been used in:
  • immunohistochemistry
  • immunoblotting
  • immunofluorescence

Actions biochimiques/physiologiques

Fused in sarcoma (FUS) plays regulatory roles in transcription, RNA splicing and transport and is implicated in multiple diseases. FUS also called translocation in liposarcoma or Tumor lysis syndrome (TLS), plays a key role in DNA repair and transcriptional regulation. Chromosomal translocation of FUS/TLS is found in human cancers and results in the production of oncogenic FUS fusion proteins. FUS is a component of inclusion bodies in patients with Huntington′s disease (HD) and spinocerebellar ataxias (SCA1) and SCA3. Mutations in the FUS gene have been identified in amyotrophic lateral sclerosis (ALS), frontotemporal lobar degeneration (FLTD) and familial amyotrophic lateral sclerosis (FALS). The majority of the FUS mutations have been identified in the C-terminal nuclear localization signal (NLS). Pathological FUS inclusions are mostly found in the cytosol of neurons and glial cells.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Tomas Smolek et al.
The Journal of comparative neurology, 524(4), 874-895 (2015-08-05)
Canine cognitive impairment syndrome (CDS) represents a group of symptoms related to the aging of the canine brain. These changes ultimately lead to a decline of memory function and learning abilities, alteration of social interaction, impairment of normal housetraining, and
Atypical Huntington?s disease with the clinical presentation of behavioural variant of frontotemporal dementia
Sutovsky S, et al.
Journal of neural transmission (Vienna, Austria : 1996), 123(12), 1423-1433 (2016)
Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment
Smolek T, et al.
The Journal of Comparative Neurology, 524(4), 874-895 (2016)
Fused in sarcoma: Properties, self-assembly and correlation with neurodegenerative diseases
Chen C, et al.
Molecules (Basel), 24(8), 1622-1622 (2019)
Hao Deng et al.
Nature reviews. Neurology, 10(6), 337-348 (2014-05-21)
The neurodegenerative diseases are a diverse group of disorders characterized by progressive loss of specific groups of neurons. These diseases affect different populations, and have a variable age of onset, clinical symptoms, and pathological findings. Variants in the FUS gene

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