S8633
Sphingomyelinase from Staphylococcus aureus
buffered aqueous glycerol solution, 100-300 units/mg protein (Lowry)
Synonyme(s) :
Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase
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About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.32
Produits recommandés
Forme
buffered aqueous glycerol solution
Niveau de qualité
Activité spécifique
100-300 units/mg protein (Lowry)
Température de stockage
2-8°C
Application
Sphingomyelinase from Staphylococcus aureus has been used to:
- induce neurotoxicity in rat cortical cultures to study the protective effects of minocycline
- determine the concentration of sphingomyelin from serum samples
- enhance sphingomyelinase activity to study PARK9-mediated exosome biogenesis
Actions biochimiques/physiologiques
Bacterial sphingomyelinase is active at neutral pH. When used in cell culture in vitro, it hydrolyzes the sphingomyelin on the outer leaflet of the plasma membrane and produces ceramide that is lipid-soluble. Sphingomyelinase is the key enzyme in the sphingomyelinase/ceramide pathway, which is implicated in the pathogenesis of several neurodegenerative disorders.
Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.
Définition de l'unité
One unit will hydrolyze 1.0 μmol of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.
Forme physique
Solution in 50% glycerol containing 0.25 M phosphate buffer, pH 7.5
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Les clients ont également consulté
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In this study, we determined whether minocycline may protect rat cortical cultures against neurotoxicity induced by sphingomyelinase/ceramide and explored the underlying mechanisms. We found that minocycline exerted strong neuroprotective effects against toxicity induced by bacterial sphingomyelinase and synthetic C2 ceramide.
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