P7181

Polyethylene glycol solution

Hybri-Max^™, 50 % (w/v), average mol wt 1,450, 0.2 μm filtered, BioReagent, suitable for hybridoma

• Synonyme(s) : PEG Solution, Polyethylene Glycol
• Numéro MDL: MFCD00081839
• Code UNSPSC : 12161902
• ID de substance PubChem : 24898829
• Nomenclature NACRES : NA.75

Propriétés

• Qualité: Hybri-Max^™
• Stérilité: 0.2 μm filtered
• Gamme de produits: BioReagent
• Forme: solution
• Poids mol.: average mol wt 1,450
• Concentration: 50 % (w/v)
• Technique(s): cell culture | hybridoma: suitable
• Impuretés: endotoxin, tested
• Température de stockage: 2-8°C
• Chaîne SMILES: C(CO)O
• InChI: 1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
• Clé InChI: LYCAIKOWRPUZTN-UHFFFAOYSA-N

Description

Description générale

Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.

Application

Polyethylene glycol solution has been used in the hybridization of the following:

• irradiated camel fibroblast cells with A23 hamster cells
• mouse splenocytes with Sp2/0-Ag 14 mouse myeloma cells
• rabbit splenocytes and cell line 240E-1

Recommended for use in a normal fusion protocol requiring 50% PEG.

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.

Actions biochimiques/physiologiques

Polyethylene glycol (PEG) is used in detergents, emulsifying agents, soaps, metal costing process, pharmaceutical preparations and cosmetics. PEG is commonly used as a lubricant. It′s application is also observed in chromatographic stationary phases. PEG prevents crystal formation in emulsions.

Conditionnement

Packaged in sealed ampules under nitrogen.

Autres remarques

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.

Reconstitution

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

Informations légales

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): 212.0 °F - closed cup
• Point d'éclair (°C): 100 °C - closed cup
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter