P7181

Polyethylene glycol solution

Hybri-Max^™, 50 % (w/v), average mol wt 1,450, 0.2 μm filtered, BioReagent, suitable for hybridoma

• Synonyme(s) : PEG Solution, Polyethylene Glycol
• MDL number: MFCD00081839
• UNSPSC Code: 12161902
• PubChem Substance ID: 24898829
• NACRES: NA.75

Propriétés

• grade: Hybri-Max^™
• Quality Segment: 200
• sterility: 0.2 μm filtered
• product line: BioReagent
• form: solution
• mol wt: average mol wt 1,450
• concentration: 50 % (w/v)
• technique(s): cell culture | hybridoma: suitable
• impurities: endotoxin, tested
• storage temp.: 2-8°C
• SMILES string: C(CO)O
• InChI: 1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
• InChI key: LYCAIKOWRPUZTN-UHFFFAOYSA-N

Description

General description

Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.

Application

Recommended for use in a normal fusion protocol requiring 50% PEG.

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.

Polyethylene glycol solution has been used in the hybridization of the following:

• irradiated camel fibroblast cells with A23 hamster cells
• mouse splenocytes with Sp2/0-Ag 14 mouse myeloma cells
• rabbit splenocytes and cell line 240E-1

Biochem/physiol Actions

Polyethylene glycol (PEG) is used in detergents, emulsifying agents, soaps, metal costing process, pharmaceutical preparations and cosmetics. PEG is commonly used as a lubricant. It′s application is also observed in chromatographic stationary phases. PEG prevents crystal formation in emulsions.

Packaging

Packaged in sealed ampules under nitrogen.

Preparation Note

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

Other Notes

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Classe de stockage: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: 212.0 °F - closed cup
• flash_point_c: 100 °C - closed cup
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter