P2860

Monoclonal Anti-PSF antibody produced in mouse

clone B92, purified from hybridoma cell culture

• Synonyme(s) : Mouse Anti-Polypyrimidine tract-binding protein-associated splicing factor
• Numéro MDL: MFCD03457573
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: B92, monoclonal
• Forme: buffered aqueous solution
• Espèces réactives: mouse, human
• Conditionnement: antibody small pack of 25 μL
• Concentration: ~2 mg/mL
• Technique(s): immunocytochemistry: suitable; immunohistochemistry (frozen sections): suitable; immunoprecipitation (IP): suitable; microarray: suitable; radioimmunoassay: suitable using cells; western blot: 2-4 μg/mL using whole extract of cultured HeLa cells
• Isotype: IgG1
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TAP1(6890)

Description

Description générale

Monoclonal Anti-PSF (mouse IgG1 isotype) is derived from the B92 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a C57BL/6J mouse immunized with a lysate of mouse bone marrow-derived stromal cell line. Polypyrimidine tract-binding protein-associated splicing factor (PSF) is an ubiquitous nuclear matrix protein. It is characterized with two consensus RNA-binding domains and an unusual amino terminus rich in proline and glutamine residues.

Immunogène

lysate of mouse bone marrow-derived stromal cell line.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunoprecipitation (1 paper)

Monoclonal Anti-PSF antibody produced in mouse has been used in:

• immunofluorescence
• immunohistochemistry
• immunoprecipitation

Actions biochimiques/physiologiques

Polypyrimidine tract-binding protein-associated splicing factor (PSF) plays a pivotal role in mammalian spliceosome. PSF is also necessary for pre-mRNA splicing and transcription. The restricted expression of PSF suggests that it is involved in the control of neuronal-specific splicing events occurring at particular stages of neuronal differentiation and maturation. Monoclonal antibodies reacting specifically with PSF are useful tools for the molecular identification and characterization of the functional activity of PSF.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Notes préparatoires

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable