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O2260

Sigma-Aldrich

Mouse Osteopontin

recombinant, expressed in NSO cells, powder, suitable for cell culture

Synonyme(s) :

Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)

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About This Item

Numéro MDL:
MFCD03100698
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

product name

Osteopontin from mouse, >95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

Source biologique

mouse

Niveau de qualité

100

Produit recombinant

expressed in NSO cells

Stérilité

sterile

Pureté

>95% (SDS-PAGE)

Forme

lyophilized powder

Poids mol.

30 kDa by SDS-PAGE (reducing)
 65 kDa by SDS-PAGE (reducing) (doublet)

Conditionnement

pkg of 50 μg

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Numéro d'accès UniProt

P10923

Conditions d'expédition

dry ice

Température de stockage

−20°C

Informations sur le gène

mouse ... Spp1(20750)

Application

Osteopontin from mouse has been used:
  • to study its role in a mouse model of preterm brain injury
  • as a positive control to analyze the relationship between the vascular calcification process and osteopontin (OPN) overexpression in the neointima of atherosclerotic plaques
  • to study its interaction on the crystal growth process of hydroxyapatite in agarose hydrogels

Actions biochimiques/physiologiques

Osteopontin (OPN) is a secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig and bovine OPN share 40% amino acid sequence identity.

Forme physique

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Remarque sur l'analyse

The biological activity is measured by its ability to mediate 293 cell adhesion.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Osteopontin upregulation in atherogenesis is associated with cellular oxidative stress triggered by the activation of scavenger receptors
Jimenez CAE, et al.
Archives of Medical Research, 43(2), 102-111 (2012)
Osteopontin as a means to cope with environmental insults: regulation of inflammation, tissue remodeling, and cell survival
Denhardt DT, et al.
The Journal of Clinical Investigation, 107(9), 1055-1061 (2001)
The effect of osteopontin and osteopontin-derived peptides on preterm brain injury
Albertsson AM, et al.
Journal of Neuroinflammation, 11(1), 197-197 (2014)
Effect of osteopontin, chondroitin sulfates (B, C), and human serum albumin on the crystallization behavior of hydroxyapatite in agarose and silica hydrogels
Jimenez CAE, et al.
Crystal Growth & Design, 8(4), 1335-1339 (2008)
Yunan Zhu et al.
Japanese journal of infectious diseases, 68(6), 467-473 (2015-04-14)
The level of osteopontin (OPN) increases during bacterial lung infection. However, the OPN level in virus-induced lung injury is unclear, and the relationship between the hyer-production of OPN and lung injury remains to be thoroughly understood. Therefore, we sought to
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