MTOX1005P24

MDR1/MRP2 Double Knockout Caco-2 Cells

human cervix, Epithelial

• Synonyme(s) : MDR1/MRP2 Double Knockout C2BBe1 Cells
• Code UNSPSC : 41106514
• Nomenclature NACRES : NA.81

Propriétés

• product name: MDR1/MRP2 Double Knockout Caco-2 Cells, one assay ready, 24 well plate
• Source biologique: human colon
• Forme: solid
• Morphologie: Epthelial
• Technique(s): drug transporter assay: suitable; permeability assay: suitable
• Application(s): ADME/TOX

Description

Description générale

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MDR1/MRP2 double knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the MDR1 and MRP2 efflux transporters.

The three week production lead time begins on the Monday following a purchase, in the third week the plates are shipped on Tuesday for receipt on Wednesday or Thursday. As a biologic product that′s shipped at room temperature the cells must be processed immediately upon receipt.

Application

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

Caractéristiques et avantages

The Caco-2 subclone, C2BBe1 cells, are ideal for transporter analysis as they express multiple transporters, are human derived and grow in a homogenous monolayer that forms tight juntions which is necessary for efflux ratio analysis. Other benefits include:

• Functional knockout of the MDR1 and MRP2 genes eliminates the reliance on chemical inhibitors to determine if a compound is an MDR1 or MRP2 substrate
• The 24 well Transwell format enables the MDR1 and MRP2 knockout cells to be included in standard drug transporter protocols
• Human assay with no interference from animal inhibitors
• Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable