Unfortunately, the product MP0040A is not optimized for mycoplasma detection as per the Guideline for Mycoplasma NAT outlined in chapter 2.6.7 of the European Pharmacopeia in terms of specificity, sensitivity/detection limit, and robustness.
MP0040A
LookOut® Mycoplasma qPCR Detection Kit
Synonyme(s) :
Quantiative PCR mycoplasma detection kit
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About This Item
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Utilisation
sufficient for 25 reactions
Niveau de qualité
Qualité
Not suitable for clinical diagnostic use.
Conditionnement
pkg of 1 kit
Conditions de stockage
dry at room temperature
Technique(s)
PCR: suitable
Application(s)
detection
microbiology
Température de stockage
2-8°C
Description générale
The LookOut Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals.
The primer/probe system detects the highly conserved 23S rRNA operon coding region of the mycoplasma genome. The kit is highly specific and does not detect eukaryotic DNA. The detection spectrum includes most mycoplasma species identified as cell culture contaminants (see Table 2 in the technical bulletin). The kit contains the nucleotide dUTP instead of dTTP and is, therefore, suitable for UNG pretreatment.
This kit already contains Taq polymerase in the Mycoplasma Mix.
The primer/probe system detects the highly conserved 23S rRNA operon coding region of the mycoplasma genome. The kit is highly specific and does not detect eukaryotic DNA. The detection spectrum includes most mycoplasma species identified as cell culture contaminants (see Table 2 in the technical bulletin). The kit contains the nucleotide dUTP instead of dTTP and is, therefore, suitable for UNG pretreatment.
This kit already contains Taq polymerase in the Mycoplasma Mix.
Application
qPCR Probe Kit for high quality and reliable quantitative detection of mycoplasma DNA in research, industrial application and product testing. Usable for direct testing of cell cultures and biologicals in combination with cell culture enrichment.
Informations légales
LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
188.6 °F
Point d'éclair (°C)
87 °C
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Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.
Protocoles
Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.
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Is this product optimized for mycoplasma detection according to the Guideline for Mycoplasma NAT outlined in chapter 2.6.7 of the European Pharmacopeia in terms of specificity, sensitivity/detection limit, and robustness?
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