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Key Documents

M9942

Sigma-Aldrich

Anticorps anti-MAP2 monoclonal antibody produced in mouse

clone HM-2, purified from hybridoma cell culture

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

HM-2, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~280 kDa

Espèces réactives

rat, chicken, human, mouse, bovine, quail

Conditionnement

antibody small pack of 25 μL

Concentration

~2 mg/mL

Technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
microarray: suitable
western blot: 1-2 μg/mL using rat brain extract

Isotype

IgG1

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... MAP2(4133)
mouse ... Mtap2(17756)
rat ... Map2(25595)

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Description générale

MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarly sized with apparent molecular weights of 280 kDa (MAP2a and b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears at levels equal to that of MAP2b. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and neurons have reached their mature morphology.
MAP2 is known to promote microtubule assembly and to form side arms on microtubules. It also interacts with neurofilaments, actin and other elements of the cytoskeleton.

Spécificité

Réagit avec toutes les formes connues de MAP2, à savoir MAP2a, MAP2b et MAP2c. Ne donne lieu à aucune réaction croisée avec les autres MAP ou la tubuline. Par coloration immunohistochimique de tissu cérébral fixé au formol et inclus en paraffine, l′anticorps assure un marquage sélectif des arbres dendritiques au sein de l′ensemble du cerveau.
This antibody recognizes all known forms of MAP2, namely MAP2a, MAP2b and MAP2c. It does not cross-react with other MAPs or tubulin. By immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue, the antibody shows selective labeling of dendritic trees throughout the brain.

Immunogène

protéines associées aux microtubules (MAP) de cerveau de rat.

Application

Anti-MAP2 antibody has been used in immunofluorescence. It has also been used in immunohistochemistry.
May be used in various immunochemical techniques including immunoblotting, immunohistochemistry, and immunocytochemistry. Monoclonals antibodies to MAP2 are used to study the cytoskeleton structure in cells.
By immunoblotting, a working antibody concentration of 1-2 μg/ml is recommended using a rat brain extract.

Actions biochimiques/physiologiques

MAP2 (microtubule-associated protein 2) expression is monitored to predict the disease progression in glioma. MAP2 is critical in the formation of dendritic protrusions in astrocytes and oligodendrocytes. In eukaryotes, MAP2 is essential for spindle assembly and chromosome segregation.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2-8 C for up to one month. For extended storage, freeze in working aliquots at 20 C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Produit(s) apparenté(s)

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Expression of EAAT2 in neurons and protoplasmic astrocytes during human cortical development
DeSilva T M, et al.
The Journal of Comparative Neurology, 520(17), 3912-3932 (2012)
The MAP2/Tau family of microtubule-associated proteins.
Dehmelt L and Halpain S
Genome Biology, 6(1), 204-204 (2005)
miR-484/MAP2/c-Myc-positive regulatory loop in glioma promotes tumor-initiating properties through ERK1/2 signaling.
Yi R, et al.
Journal of Molecular Histology, 49(2) (2018)
Remarkable reduction of MAP2 in the brains of scrapie-infected rodents and human prion disease possibly correlated with the increase of calpain.
Guo Y, et al.
PLoS ONE, 7, e30163-e30163 (2012)
Promotion of neuronal differentiation of neural progenitor cells by using EGFR antibody functionalized collagen scaffolds for spinal cord injury repair
Li X, et al.
Biomaterials, 34(21), 5107-5116 (2013)

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